Metabolomics analysis reveals the response mechanism to carbonate alkalinity toxicity in the gills of Eriocheir sinensis

文献类型： 外文期刊

第一作者： Zhang, Rui

作者： Zhang, Rui;Zhao, Zhigang;Li, Mingshuai;Luo, Liang;Wang, Shihui;Guo, Kun;Xu, Wei;Li, Mingshuai;Zhang, Rui;Zhao, Zhigang;Luo, Liang;Wang, Shihui;Guo, Kun;Xu, Wei;Zhao, Zhigang

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关键词： Eriocheir sinensis; Gills; Carbonate alkalinity; Metabolomics

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：4.52； 五年影响因子：4.203 ）

ISSN： 1532-0456

年卷期： 2023 年 263 卷

页码：

收录情况： SCI

摘要： Aquatic water with carbonate alkalinity presents a survival challenge to aquatic animals. As an economically important crab, large quantities of Eriocheir sinensis are cultured in carbonate-type saline-alkali ponds, while the toxic effect on E. sinensis from carbonate alkalinity is still unclear. In this study, untargeted liquid chromatography-mass spectrometry metabolomics was performed to investigate the metabolic change caused by culture alkalinity, and confirmed distinct physiological response under gradient alkalinities. There were 39 differential metabolites obtained in the low-alkalinity group (4.35 mmol/L) versus control group, and "arach-idonic acid metabolism" was enriched as a core response pathway. 93 differential metabolites were identified in the high-alkalinity group (17.43 mmol/L) versus control group, and a complex response net was manifested through integrated analysis, building by "steroid hormone biosynthesis", "phenylalanine, tyrosine and trypto-phan biosynthesis", "phosphonate and phosphinate metabolism", "phenylalanine metabolism", "mineral ab-sorption", "purine metabolism" and "carbon metabolism". This indicated the mobilization of energy reserves and the suppression of protein and amino acid catabolism were manifested in E. sinensis gills to defense high alka-linity stress. In addition, the persistently regulation of key metabolites under various alkalinity, including diuretic compound "spironolactone" and the antiphlogistic compound "LXB4", suggested anti-inflammatory ac-tion and excretion regulation were initiated to defend the stress.

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