A prolific and robust whole-genome genotyping method using PCR amplification via primer-template mismatched annealing

文献类型： 外文期刊

第一作者： Zhao, Sheng

作者： Zhao, Sheng;Wang, Liqun;Luo, Minxuan;Zhi, Haijian;Zhao, Sheng;Zhang, Cuicui;Luo, Minxuan;Zhang, Peng;Wang, Yue;Malik, Waqar Afzal;Chen, Peng;Lu, Hong;Xiang, Yong;Liu, Yuwen;Ruan, Jue;Qian, Qian;Chang, Yuxiao;Qiu, Xianjin;Wang, Chongrong

作者机构：

关键词： background selection; foreground genotyping; primer-template mismatched annealing; marker-assisted breeding; whole-genome genotyping

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年 65 卷 3 期

页码：

收录情况： SCI

摘要： Whole-genome genotyping methods are important for breeding. However, it has been challenging to develop a robust method for simultaneous foreground and background genotyping that can easily be adapted to different genes and species. In our study, we accidently discovered that in adapter ligation-mediated PCR, the amplification by primer-template mismatched annealing (PTMA) along the genome could generate thousands of stable PCR products. Based on this observation, we consequently developed a novel method for simultaneous foreground and background integrated genotyping by sequencing (FBI-seq) using one specific primer, in which foreground genotyping is performed by primer-template perfect annealing (PTPA), while background genotyping employs PTMA. Unlike DNA arrays, multiple PCR, or genome target enrichments, FBI-seq requires little preliminary work for primer design and synthesis, and it is easily adaptable to different foreground genes and species. FBI-seq therefore provides a prolific, robust, and accurate method for simultaneous foreground and background genotyping to facilitate breeding in the post-genomics era.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序