文献类型： 外文期刊
第一作者： Li, P.
作者： Li, P.;Rodina, M.;Hulak, M.;Gela, D.;Li, Z. H.;Linhart, O.;Li, P.;Li, Z. H.
作者机构：
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN： 0175-8659
年卷期： 2010 年 26 卷 5 期
页码：
收录情况： SCI
摘要： P>Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 +/- 5.63 to 105.78 +/- 5.27 mu ms-1) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 +/- 0.16 mg ml-1), spermatozoa concentration (0.28 +/- 0.27 x 109 spz ml-1), seminal plasma osmolality (51.33 +/- 4.91 mOsmol kg-1), the pH (8.49 +/- 0.01) and Na+ (18.97 +/- 3.65 mm)(,) K+ (2.83 +/- 1.36 mm), Ca2+ (0.19 +/- 0.06 mm), Mg2+ (0.49 +/- 0.23 mm) and Cl- (6.33 +/- 0.58 mm) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and iso-electric points ranging from 5 to 9.5 were found. The observed physiological and biochemical properties, together with protein patterns, should be considered for the development of methods for controlled reproduction and sperm cryopreservation for the highly endangered beluga sturgeon.
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