The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii
文献类型: 外文期刊
第一作者: Wang, Jin-Lei
作者: Wang, Jin-Lei;Li, Ting-Ting;Liang, Qin-Li;Zhang, Zhi-Wei;Wang, Meng;Elsheikha, Hany M.;Sibley, L. David;Zhu, Xing-Quan
作者机构:
期刊名称:NATURE COMMUNICATIONS ( 影响因子:16.6; 五年影响因子:17.0 )
ISSN:
年卷期: 2022 年 13 卷 1 期
页码:
收录情况: SCI
摘要: Phenotypic switching between tachyzoite and bradyzoite is the fundamental mechanism underpinning the pathogenicity and adaptability of the protozoan parasite Toxoplasma gondii. Although accumulation of cytoplasmic starch granules is a hallmark of the quiescent bradyzoite stage, the regulatory factors and mechanisms contributing to amylopectin storage in bradyzoites are incompletely known. Here, we show that T. gondii protein phosphatase 2A (PP2A) holoenzyme is composed of a catalytic subunit PP2A-C, a scaffold subunit PP2A-A and a regulatory subunit PP2A-B. Disruption of any of these subunits increased starch accumulation and blocked the tachyzoite-tobradyzoite differentiation. PP2A contributes to the regulation of amylopectin metabolism via dephosphorylation of calcium-dependent protein kinase 2 at S679. Phosphoproteomics identified several putative PP2A holoenzyme substrates that are involved in bradyzoite differentiation. Our findings provide novel insight into the role of PP2A as a key regulator of starch metabolism and bradyzoite differentiation in T. gondii.
分类号:
- 相关文献
作者其他论文 更多>>
-
Environmental risk thresholds and prediction models of Cd in Chinese agricultural soils
作者:Yu, Lei;Chen, Shibao;Wang, Jing;Qin, Luyao;Sun, Xiaoyi;Wang, Meng;Zhang, Xing;Zhang, Xing;Chen, Shibao;Wang, Meng;Zhang, Xing
关键词:Soil Cd; Soil environmental risk threshold; Derivation methodology; Hormesis; SSD; Hc3
-
Comammox dominate soil nitrification under different N fertilization regimes in semi-arid areas of Northeast China
作者:Feng, Xuewan;Tan, Pan;Liu, Hang;Li, Cuilan;Zhang, Jinjing;Wang, Meng;Li, Qian;Qin, Yubo;Sun, Bo
关键词:N fertilization gradient; Comammox; AOA; AOB; Nitrification contribution rate
-
Stereoselective study on chiral fungicide metconazole in four kinds of fruits: Absolute configuration, SFC-MS/MS enantioseparation, degradation and risk assessment
作者:Diao, Ziyang;Zhang, Chenghui;Diao, Ziyang;Di, Shanshan;Qi, Peipei;Liu, Zhenzhen;Wang, Zhiwei;Zhao, Huiyu;Wang, Xinquan;Diao, Ziyang;Di, Shanshan;Qi, Peipei;Liu, Zhenzhen;Wang, Zhiwei;Zhao, Huiyu;Wang, Xinquan;Wang, Meng;Zhang, Chenghui;Wang, Meng
关键词:Chiral metconazole; Absolute configuration; SFC-MS/MS enantioseparation; Fruits; Stereoselective degradation
-
PIN5 is involved in regulating NH4+ efflux and primary root growth under high-ammonium stress via mediating intracellular auxin transport
作者:Di, Dong-Wei;Ma, Mingkun;Li, Guangjie;Wang, Meng;Shi, Weiming;Wu, Jingjing;Ma, Mingkun;Shi, Weiming;Kronzucker, Herbert J.
关键词:PIN5; Ammonium; Intracellular auxin homeostasis; H+ fluxes; NH4+ fluxes
-
Ecological risk threshold for Pb in Chinese soils
作者:Qin, Luyao;Sun, Xiaoyi;Yu, Lei;Wang, Jing;Wang, Meng;Chen, Shibao;Modabberi, Soroush
关键词:Lead; Hormesis; Toxicity threshold; Species sensitivity distribution (SSD); Hazard concentration for 5% of species(HC5)
-
Functional characterization of powdery mildew resistance-related genes HbSGT1a and HbSGT1b in Hevea brasiliensis Muell. Arg.
作者:Wang, Meng;Xiao, Huaxing;Yang, Ye;Yu, Haiyang;Zhang, Yu;Li, Xiaona;Wan, Sanlian;Qin, Bi;Qin, Bi
关键词:Rubber tree; HbSGT1; Powdery mildew; Abiotic stress; Hormone signalling
-
Salt stress-induced changes in soil metabolites promote cadmium transport into wheat tissues
作者:Wang, Lifu;Qin, Luyao;Sun, Xiaoyi;Zhao, Shuwen;Yu, Lei;Chen, Shibao;Wang, Meng
关键词:Saline stress; Cadmium availability; Wheat growth; Metabolite profile; Rhizosphere