Effects of Insulin on Proliferation, Apoptosis, and Ferroptosis in Primordial Germ Cells via PI3K-AKT-mTOR Signaling Pathway
文献类型: 外文期刊
第一作者: Ye, Liu
作者: Ye, Liu;Liu, Xin;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Chen, Guohong;Li, Bichun;Ye, Liu;Liu, Xin;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Chen, Guohong;Li, Bichun;Ye, Liu;Liu, Xin;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Chen, Guohong;Li, Bichun;Song, Jiuzhou;Han, Wei;Li, Bichun
作者机构:
关键词: insulin; PGCs; proliferation; apoptosis; ferroptosis; PI3K-AKT-mTOR
期刊名称:GENES ( 影响因子:3.5; 五年影响因子:3.9 )
ISSN:
年卷期: 2023 年 14 卷 10 期
页码:
收录情况: SCI
摘要: Primordial germ cells (PGCs) are essential for the genetic modification, resource conservation, and recovery of endangered breeds in chickens and need to remain viable and proliferative in vitro. Therefore, there is an urgent need to elucidate the functions of the influencing factors and their regulatory mechanisms. In this study, PGCs collected from Rugao yellow chicken embryonic eggs at Day 5.5 were cultured in media containing 0, 5, 10, 20, 50, and 100 mu g/mL insulin. The results showed that insulin regulates cell proliferation in PGCs in a dose-dependent way, with an optimal dose of 10 mu g/mL. Insulin mediates the mRNA expression of cell cycle-, apoptosis-, and ferroptosis-related genes. Insulin at 50 mu g/mL and 100 mu g/mL slowed down the proliferation with elevated ion content and GSH/oxidized glutathione (GSSG) in PGCs compared to 10 mu g/mL. In addition, insulin activates the PI3K/AKT/mTOR pathway dose dependently. Collectively, this study demonstrates that insulin reduces apoptosis and ferroptosis and enhances cell proliferation in a dose-dependent manner via the PI3K-AKT-mTOR signaling pathway in PGCs, providing a new addition to the theory of the regulatory role of the growth and proliferation of PGC in vitro cultures.
分类号:
- 相关文献
作者其他论文 更多>>
-
The apple lipoxygenase MdLOX3 positively regulates zinc tolerance
作者:Chen, Guo-Lin;Wang, Da-Ru;Liu, Xin;Wang, Xun;Liu, Hao-Feng;Zhang, Zhen-Lu;You, Chun-Xiang;Li, Lin-Guang;Zhang, Chun-Ling
关键词:Heavy metal; Apple; Lipoxygenase; MdLOX3; Zinc stress tolerance; Scavenging by activated oxygen
-
miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene
作者:Zhou, Kai-Zhi;Wu, Peng-Fei;Ling, Xuan-Ze;Zhang, Jin;Wang, Qi-Fan;Zhang, Xin-Chao;Zhang, Tao;Zhang, Gen-Xi;Xue, Qian;Han, Wei
关键词:miRNA; skeletal muscle; growth and development; chicken
-
Antifungal activities of metconazole against the emerging wheat pathogen Fusarium pseudograminearum
作者:Liu, Xin;Wang, Shuang;Wang, Liwen;Shi, Jianrong;Xu, Jianhong;Liu, Xin;Wang, Shuang;Wang, Liwen;He, Dan;Shi, Jianrong;Gao, Tao;Xu, Jianhong;Wu, Jiawen;Fan, Zhongyue;Mohamed, Sherif Ramzy;Dawood, Dawood H.
关键词:Fusarium pseudograminearum; Metconazole; Fusarium crown rot; Sensitivity; RNA-Seq; Control efficacy
-
The GATA transcription factor TaGATA1 recruits demethylase TaELF6-A1 and enhances seed dormancy in wheat by directly regulating TaABI5
作者:Wei, Xuening;Li, Yuyan;Zhu, Xiuliang;Liu, Xin;Ye, Xingguo;Zhang, Zengyan;Zhou, Miaoping
关键词:ABSCISIC ACID INSENSITIVE5 (ABI5); common wheat; demethylase; GATA transcription factor; preharvest sprouting; seed dormancy
-
Floral scent components of the hybrids between Lagerstroemia fauriei and Lagerstroemia 'Tuscarora'
作者:Zhou, Ting;Zhou, Yanwei;Li, Yunlong;Wang, Chuanyong;Zhang, Fan;Chong, Xinran;Chen, Hong;Ning, Kun;Han, Wei;Zhang, Donglin
关键词:Lagerstroemia spp; Floral scent; Characteristic compounds; HS-SPME-GC-MS; Genetic rule; Targeted breeding
-
Hydrogen sulfide, regulated by VvWRKY30, promotes berry color changes in grapevine cabernet sauvignon
作者:Liu, Xin;Pei, Yuncheng;Zhu, Dan;Pei, Yuncheng;Cheng, Fansheng;Wang, Chaoping
关键词:H2S; Berry color change; VvWRKY30; Cabernet Sauvignon; Veraison
-
Muscle fiber plasticity, stress physiology, and muscle transcriptome determine the inter-individual difference of swimming performance in the large yellow croaker (Larimichthys crocea)
作者:Li, Siping;Liu, Xin;Lin, Tingting;Feng, Guangpeng;Wang, Xiaoshan;Zhang, Dong;Li, Siping;Liu, Xin;Lin, Tingting;Feng, Guangpeng;Wang, Xiaoshan;Zhang, Dong
关键词:The large yellow croaker; Swimming performance; Inter -individual difference; Muscle transcriptome; Stress physiology