Baicalein targets CD36 to prevent foam cell formation by suppressing the excessive uptake of oxLDL and accelerating ABCA1-mediated cholesterol efflux in oxLDL-induced THP-1 macrophages

文献类型： 外文期刊

第一作者： Li, Jingda

作者： Li, Jingda;Xiong, Tao;Wang, Tianqi;Wang, Meng;Wang, Chao;Sun, Wenlong;Yang, Fuyuan;Wang, Xudong;Tan, Zhijian

作者机构：

关键词： Baicalein; CD36; Oxidized low -density lipoprotein; ABCA1; Cholesterol efflux; Macrophage foam cell formation

期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：5.223； 五年影响因子：5.178 ）

ISSN： 1756-4646

年卷期： 2022 年 97 卷

页码：

收录情况： SCI

摘要： Oxidized low-density lipoprotein (oxLDL)-induced macrophage foam cell formation plays an important role in atherosclerosis progression. Baicalein is a constituent of Scutellaria baicalensis. Herein, we investigated the effect of baicalein on macrophage foam cell formation and the underlying mechanism. The THP-1 macrophage foam cell was established by oxLDL stimulation and the effects of baicalein on cholesterol uptake and efflux were analysed by molecular docking, ELISA, immunofluorescence, western blot, the inhibitor and siRNA experiment. The results showed that baicalein suppressed oxLDL-induced cholesterol accumulation. Mechanistically, baica-lein reduced oxLDL uptake through competitive inhibiting the binding of CD36 to the epitope structure of oxLDL. Moreover, the binding of baicalein to CD36 enhanced the cholesterol efflux via the CD36-Src-JNK-ABCA1 sig-nalling pathway. Meanwhile, Src and JNK inhibitors reversed the baicalein-induced reduction in cholesterol accumulation. In conclusion, these findings suggested that baicalein as a food supplement could inhibit macrophage foam cell formation and play an anti-atherosclerosis effect.

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