Effect of shading on physiological attributes and comparative transcriptome analysis of Camellia sinensis cultivar reveals tolerance mechanisms to low temperatures
文献类型: 外文期刊
第一作者: Zaman, Shah
作者: Zaman, Shah;Shen, Jiazhi;Wang, Shuangshuang;Ding, Zhaotang;Song, Dapeng;Wang, Hui;Ding, Shibo;Pang, Xu;Wang, Mengqi;Sabir, Irfan Ali;Wang, Yu;Ding, Zhaotang
作者机构:
关键词: shading; tea; physiological attributes; transcriptome analysis; low temperature; signal transductions
期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:5.6; 五年影响因子:6.8 )
ISSN: 1664-462X
年卷期: 2023 年 14 卷
页码:
收录情况: SCI
摘要: Tea is a vital beverage crop all over the world, including in China. Low temperatures restrict its growth, development, and terrestrial distribution, and cold event variability worsens cold damage. However, the physiological and molecular mechanisms of Camellia sinensis under shade in winter remain unclear. In our study, tea leaves were utilized for physiological attributes and transcriptome analysis in November and December in three shading groups and no-shade control plants. When compared to the no-shade control plants, the shading group protected tea leaves from cold damage, increased photochemical efficiency (Fv/Fm) and soil plant analysis development (SPAD), and sustained chlorophyll a, chlorophyll b, chlorophyll, and carotenoid contents by physiological mean. Then, transcriptome analysis revealed 20,807 differentially expressed genes (DEGs) and transcription factors (TFs) in November and December. A comparative study of transcriptome resulted in 3,523 DEGs and many TFs under SD0% vs. SD30%, SD0% vs. SD60%, and SD0% vs. SD75% of shading in November and December. Statistically, 114 DEGs were downregulated and 72 were upregulated under SD0% vs. SD30%. SD0% vs. SD60% resulted in 154 DEGs, with 60 downregulated and 94 upregulated. Similarly, there were 505 DEGs of which 244 were downregulated and 263 were upregulated under SD0% vs. SD75% of shading throughout November. However, 279 DEGs were downregulated and 105 were upregulated under SD0% vs. SD30%. SD0% vs. SD60% resulted in 296 DEGs, with 172 downregulated and 124 upregulated. Finally, 2,173 DEGs were regulated in December, with 1,428 downregulated and 745 upregulated under SD0% vs. SD75%. These indicate that the number of downregulated DEGs in December was higher than the number of upregulated DEGs in November during low temperatures. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of differentially expressed genes were highly regulated in the photosynthesis, plant hormone signal transduction, and mitogen-activated protein kinase (MAPK) signaling pathways. However, qRT-PCR and RNA-seq relative expression of photosynthetic (DEGs) Lhcb2 in both November and December, plant hormone (DEGs) BRI1 and JAZ in November and IAA and ERF1 in December, and key DEGs of MAPK signal transduction FLS2, CHIB, and MPK4 in November and RBOH, MKK4_5, and MEKK1 in December in three shading groups and no-shade control plants responded to tea cold tolerance. The enhanced expression of light-harvesting photosystem I gene Lhca5, light-harvesting photosystem II gene Lhcb2, and mitogen-activated protein kinases MEKK1 and MPK4/6 enhance the cold-tolerance mechanism of C. sinensis. These comprehensive transcriptomic findings are significant for furthering our understanding of the genes and underlying regulatory mechanisms of shade-mediated low-temperature stress tolerance in horticultural crops.
分类号:
- 相关文献
作者其他论文 更多>>
-
Self-cleaning paper-based microfluidic biosensor employing DNAzyme and semiconducting single-walled carbon nanotube for copper ion detection
作者:Hou, Xiaopeng;Wang, Hui;Cheng, Qiongyi;Wang, Hui
关键词:Copper pollution; Chemiresistive; Superhydrophobic paper; Paper-based electrode; Electrochemical biosensor
-
A dual-RPA based lateral flow strip for sensitive, on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops
作者:Wang, Jinbin;Wang, Yu;Hu, Xiuwen;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Jinbin;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Yu;Liu, Xiaofeng;Liu, Juan;Zhu, Lemei;Liu, Juan;Zhu, Lemei;Zeng, Haijuan;Liu, Hua
关键词:Genetically modified crops; On-site detection; Lateral flow test strips; Dual recombinase polymerase amplification; (RPA)
-
Insight into the molecular mechanism underlying the enhancement of antioxidant activity in ovalbumin by high-energy electron beam irradiation
作者:Chen, Haiqi;Bian, Zhongyue;Wen, Pingwei;Wang, Hui;Hu, Yueming;Tu, Zongcai;Hu, Yueming;Tu, Zongcai;Liu, Guangxian;Hu, Yueming;Wang, Hui;Liu, Guangxian
关键词:Ovalbumin; Antioxidant activity; Irradiation; Oxidation sites; Mass spectrometry
-
Changes of atrazine dissipation and microbial community under coexistence of graphene oxide in river water
作者:Wang, Qinghai;Peng, Lei;Wang, Peixin;Zhou, Zixin;Li, Cui;Peng, Lei;Zhou, Zixin;Chen, Chuansheng;Wang, Yu
关键词:Pesticide pollution; Graphene oxide; Microbial diversity; Atrazine fate; Aquatic ecosystems
-
Dual-grafted dextran based nanomicelles: Higher antioxidant, anti-inflammatory and cellular uptake efficiency for quercetin
作者:He, Zuyu;Liu, Yunhao;Wang, Hui;Li, Puwang;Wang, Chao;Zhou, Chuang;Song, Shuhui;Chen, Shaohua;Yang, Ziming;Wang, Hui;Chen, Yu;Yang, Ziming;Huang, Guocong
关键词:Dextran; Nanomicelles; Antioxidant and anti-inflammatory
-
Long-term non-phosphorus application increased paddy methane emission by promoting organic acid and methanogen abundance in Tai Lake region, China
作者:Xu, Chen;Shen, Susu;Feng, Yuanyuan;He, Zhu;Mishra, Tripti;Xue, Lihong;Xu, Chen;Xue, Lihong;Shen, Susu;He, Zhu;Xue, Lihong;Zhou, Beibei;Shi, Linlin;Wang, Haihou;Wang, Yu;Xue, Lihong
关键词:Phosphorus; Paddy field; Methane emission; Methanogen; Methanotroph
-
AtMCM10 promotes DNA replication-coupled nucleosome assembly in Arabidopsis
作者:Zhao, Xinjie;Jin, Dan;Cheng, Jinkui;Chen, Hui;Li, Zhen;Wang, Yu;Gong, Zhizhong;Wang, Jingyi;Lou, Huiqiang;Zhu, Jian-Kang;Du, Xuan;Du, Xuan;Gong, Zhizhong
关键词:AtMCM10; nucleosomes assembly