Analysis of the C2H2 Gene Family in Maize (Zea mays L.) under Cold Stress: Identification and Expression
文献类型: 外文期刊
第一作者: Li, Sinan
作者: Li, Sinan;Li, Yunlong;Cai, Quan;Li, Xin;Sun, Yan;Yu, Tao;Yang, Jianfei;Zhang, Jianguo
作者机构:
关键词: maize (Zea mays L.); cold stress; C2H2 gene family
期刊名称:LIFE-BASEL ( 影响因子:3.2; 五年影响因子:3.2 )
ISSN:
年卷期: 2023 年 13 卷 1 期
页码:
收录情况: SCI
摘要: The C2H2 zinc finger protein is one of the most common zinc finger proteins, widely exists in eukaryotes, and plays an important role in plant growth and development, as well as in salt, low-temperature, and drought stress and other abiotic stress responses. In this study, C2H2 members were identified and analyzed from the low-temperature tolerant transcriptome sequencing data of maize seedlings. The chromosome position, physical and chemical properties, evolution analysis, gene structure, conservative motifs, promoter cis elements and collinearity relationships of gene the family members were analyzed using bioinformatics, and the expression of the ZmC2H2 gene family under cold stress was analyzed by fluorescent quantitative PCR. The results showed that 150 members of the C2H2 zinc finger protein family were identified, and their protein lengths ranged from 102 to 1223 bp. The maximum molecular weight of the ZmC2H2s was 135,196.34, and the minimum was 10,823.86. The isoelectric point of the ZmC2H2s was between 33.21 and 94.1, and the aliphatic index was 42.07-87.62. The promoter cis element analysis showed that the ZmC2H2 family contains many light-response elements, plant hormone-response elements, and stress-response elements. The analysis of the transcriptome data showed that most of the ZmC2H2 genes responded to cold stress, and most of the ZmC2H2 genes were highly expressed in cold-tolerant materials and lowly expressed in cold-sensitive materials. The real-time quantitative PCR (qRT-PCR) analysis showed that ZmC2H2-69, ZmC2H2-130, and ZmC2H2-76 were significantly upregulated, and that ZmC2H2-149, ZmC2H2-33, and ZmC2H2-38 were significantly downregulated. It is hypothesized that these genes, which function in different metabolic pathways, may play a key role in the maize cold response. These genes could be further studied as candidate genes. This study provides a theoretical reference for further study on the function analysis of the maize C2H2 gene family.
分类号:
- 相关文献
作者其他论文 更多>>
-
The responses of soil Cd fractions to disinfestation regimes associated with microbial communities
作者:Li, Xin;Tao, Yu;Chen, Fang;Tan, Fangjun;Zhou, Chi;Chen, Jie;Xue, Tao;Xie, Yunhe;Chen, Jie;Xue, Tao;Xie, Yunhe;Li, Xin;Li, Xin;Tao, Yu;Chen, Fang;Tan, Fangjun;Zhou, Chi;Chen, Jie;Xue, Tao;Xie, Yunhe;Chen, Jie;Xue, Tao;Xie, Yunhe
关键词:Cadmium immobilization; Disinfestation regimes; Microbial community; Reductive soil disinfestation technology
-
Spatial-temporal distribution of deoxynivalenol, aflatoxin B1, and zearalenone in the solid-state fermentation basin of traditional vinegar and their potential correlation with microorganisms
作者:Shen, Lingqin;Wang, Yifan;Li, Xin;Hou, Ziqing;Shi, Jiyong;Battino, Maurizio;Routledge, Michael N.;Zou, Xiaobo;Zhang, Di;Mao, Jin;Battino, Maurizio;Battino, Maurizio;Routledge, Michael N.;Gong, Yunyun
关键词:Mycotoxin; Chinese vinegar; Solid-state fermentation; Lactobacillus; Aspergillus
-
Effect of Lactobacillus salivarius SNK-6 on egg quality, intestinal morphology, and cecal microbial community of laying hens
作者:Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Yan, Huaxiang
关键词:Lactobacillus salivarius; egg quality; intestinal morphology; cecal microbial community; laying hen
-
Cloning and expression study of a high-affinity nitrate transporter gene from Zea mays L
作者:Li, Guoliang;Wang, Mingquan;Yang, Jianfei;Hu, Guanghui;Chang, Xu;Dong, Yi;Shumei, Jin;Dong, Yi;Hu, Guanghui;Shumei, Jin
关键词:Zea mays; High-affinity nitrate transporter gene; salt shock
-
Non-Destructive Detection of Meat Quality Based on Multiple Spectral Dimension Reduction Methods by Near-Infrared Spectroscopy
作者:Zheng, Xiaochun;Chen, Li;Li, Xin;Zhang, Dequan
关键词:non-destructive detection; meat quality; dimension reduction; near-infrared spectroscopy
-
Comparison of Rhizospheric and Endophytic Bacterial Compositions between Netted and Oriental Melons
作者:Xiao, Jian;Sun, Yan;Tang, Xiaofu;Yang, Shangdong;He, Yi;Huang, Jinyan;Xiao, Jian
关键词:netted melon (var; reticulatus Naud; ); oriental melon (var; conomon Makino); endophytic bacteria; high-throughput sequencing; rhizosphere
-
Characterization of flavor perception and characteristic aroma of traditional dry-cured fish by flavor omics combined with multivariate statistics
作者:Chen, Fangxue;Shen, Lingwei;Qiao, Yu;Wu, Wenjin;Xiong, Guangquan;Wang, Lan;Li, Xin;Ding, Anzi;Shi, Liu;Chen, Fangxue;Shen, Lingwei;Shi, Xujia;Deng, Yi;Qiao, Yu;Wu, Wenjin
关键词:Dry-cured fish; GC-O-MS; Recombination and omission experiments; Odor activity values; Key odorant