E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis

文献类型： 外文期刊

第一作者： Li, Jingxuan

作者： Li, Jingxuan;Wang, Jiying;Wang, Yanping;Zhao, Xueyan;Su, Tao

作者机构：

关键词： lncRNA; Super-enhancer; Hepatocellular carcinoma; E2F1

期刊名称：CLINICAL EPIGENETICS （ 影响因子：5.7； 五年影响因子：6.7 ）

ISSN： 1868-7075

年卷期： 2023 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Introduction Super-enhancer-associated lncRNAs play important roles in the occurrence and development of malignant tumors, including hepatocellular carcinoma (HCC).Objectives The current work aimed to identify and characterize super-enhancer-associated lncRNAs in the pathogenesis of HCC.Methods H3K27ac ChIP-seq data from HepG2 cell line and two HCC tissues were used to identify super-enhancer-associated lncRNAs in HCC. JQ-1 treatment and CRISPR-dCas9 system were performed to confirm super-enhancer activity. Quantitative real-time PCR (qPCR), ChIP-qPCR, and dual-luciferase reporter system assay demonstrated the regulation of E2F1 on super-enhancer. Functional loss experiment was used to identify the function of LINC01004.Results In this study, we identified and characterized LINC01004, a novel super-enhancer-associated lncRNA, as a crucial oncogene in HCC. LINC01004 was upregulated in liver cancer tissues and was associated with poor patient prognosis. Moreover, LINC01004 promoted cell proliferation and metastasis of HCC. The binding of E2F1 to the super-enhancer could promote the transcription of LINC01004, while the inhibition of super-enhancer activity decreased LINC01004 expression.Conclusion This finding might provide mechanistic insights into the molecular mechanisms underlying hepatocarcinogenesis and the biological function of super-enhancer. LINC01004 can serve as a potential therapeutic target for HCC patient.

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