miR-34a Regulates the Activity of HIF-1a and P53 Signaling Pathways by Promoting GLUT1 in Genetically Improved Farmed Tilapia (GIFT,Oreochromis niloticus) Under Hypoxia Stress

文献类型： 外文期刊

第一作者： Qiang, Jun

作者： Qiang, Jun;Zhu, Xiao-Wen;Qiang, Jun;He, Jie;Tao, Yi-Fan;Bao, Jin-Wen;Zhu, Jun-Hao;Xu, Pao

作者机构：

关键词： hypoxia stress; genetically improved farmed tilapia; miR-34a; GLUT1; apoptosis

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）

ISSN： 1664-042X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： In fish under hypoxia stress, homeostasis can become imbalanced, leading to tissue and organ damage and decreased survival. Therefore, it is useful to explore the molecular and physiological regulation mechanisms that function in fish under hypoxia stress. The microRNA miR-34a is involved in fat and glycogen metabolism, and in apoptosis. In this study, we first verified thatGLUT1, the gene encoding glucose transporter 1, is a potential target gene of miR-34a in genetically improved farmed tilapia (GIFT,Oreochromis niloticus) by dual luciferase reporter assays. Then, we clarified the regulatory relationship between miR-34a andGLUT1by qRT-PCR analyses. We analyzed the regulatory effects of knockdown or promotion ofGLUT1expressionin vitroandin vivoin GIFT under hypoxia stress. The results confirm thatGLUT1is a target gene of miR-34a in GIFT. Down-regulation of miR-34a significantly promotedGLUT1expression. Knockdown ofGLUT1reduced the glycogen content in GIFT liver cells, inhibitedHIF-1agene expression, up-regulated the expression of genes involved in P53 signaling pathways (P53andCASPASE-3genes), and accelerated hepatocyte apoptosis under hypoxia stress. Compared with the control group, the group injected in the tail vein with miR-34a antagomir showed up-regulated expression ofGLUT1in the liver, increased liver glycogen content at 96 h of hypoxia stress, down-regulated expression ofP53andCASPASE-3, and decreased serum aspartate aminotransferase and alanine aminotransferase enzyme activities. Our results provide information about the molecular regulation mechanism of miRNAs and their target genes in fish during the response to hypoxia stress.

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