Efficiency of chemotherapy combined with thermotherapy for eliminating grapevine leafroll-associated virus 3 (GLRaV-3)
文献类型: 外文期刊
第一作者: Hu, Guojun
作者: Hu, Guojun;Dong, Yafeng;Zhang, Zunping;Fan, Xudong;Ren, Fang
作者机构:
关键词: In vitro grapevine; Grapevine leafroll-associated virus 3 (GLRaV-3); Ribavirin; Thermotherapy; Virus elimination
期刊名称:SCIENTIA HORTICULTURAE ( 影响因子:3.463; 五年影响因子:3.672 )
ISSN: 0304-4238
年卷期: 2020 年 271 卷
页码:
收录情况: SCI
摘要: Grapevine leafroll-associated virus 3 (GLRaV-3) is distributed in all grapevine-growing regions worldwide, resulting in significant economic losses. In this study, ribavirin (15 and 25 mu g/mL, R15 and R25), high temperature (37 degrees C, T), and a combined treatment (R15 + T and R25 + T) were used to eliminate the virus from in vitro GLRaV-3-infected Vitis champini plantlets. The phytotoxicity of ribavirin on plantlets was found in R15 and R25 lasted 30 days, but the damage of high temperature on grapevine plantlets was found in T, R15 + T and R25 + T at the beginning of treatment. Moreover, high temperature could enhance the growth of grapevine plantlets. The survival rate was lowest for the regenerated T plants (23.3%). In contrast, the survival rates for the regenerated plants that underwent the chemotherapy (R15 and R25) and the combined treatment (R15 + T and R25 + T) were 47.0% (31/66) and 41.0% (50/122), respectively. Reverse transcription polymerase chain reaction and quantitative real-time PCR assays were used to evaluate the efficiency of the GLRaV-3 elimination in the regenerated grapevine plants. The combined treatment was most effective for eliminating GLRaV-3. Specifically, GLRaV-3 was detected in all regenerated plants after the 40-day R15 and R25 treatments, whereas the virus elimination rates for the 20-day R15 + T and R25 + T treatments exceeded 80%. Additionally, the virus elimination rate for the regenerated T plants was 42.9% for the same treatment periods.
分类号:
- 相关文献
作者其他论文 更多>>
-
Identification and complete genome sequence of a novel sadwavirus discovered in chrysanthemum (Chrysanthemum morifolium Ramat.)
作者:Chen, Jie;Wang, Hui;Cao, Kuirong;Dong, Yafeng;Fan, Xudong;Zhang, Jie;Ma, Changnian;Cao, Liangliang;Shen, Leiding
关键词:
-
Integrated Transcriptome and Metabolome Dissecting Interaction between Vitis vinifera L. and Grapevine Fabavirus
作者:Zhang, Baodong;Zhang, Mengyan;Jia, Xiaojun;Hu, Guojun;Ren, Fang;Fan, Xudong;Dong, Yafeng
关键词:GFabV concentration; plant defense; metabolome; transcriptome; grapevine
-
Genomic characterization of a novel torradovirus infecting Arctium lappa L. in China
作者:Li, Zhengnan;Zhang, Lei;Song, Shuang;Fan, Xudong;Liang, Qiaolan;Chen, Yahan;Li, Minhui
关键词:
-
Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses
作者:Hu, Guojun;Dong, Yafeng;Zhang, Zunping;Fan, Xudong;Ren, Fang
关键词:grapevine; long shoot tip culture; grapevine viruses; virus variant structure; viral titer
-
Detection and genetic variation analysis of apple chlorotic leaf spot virus and apple stem grooving virus in wild apple (Malus sieversii) from Xinjiang, China
作者:Jia, Xiao-Jun;Zhang, Zun-Ping;Fan, Xu-Dong;Ren, Fang;Dong, Ya-Feng;Hu, Guo-Jun
关键词:apple chlorotic leaf spot virus; apple stem grooving virus; genetic variation; Malus sieversii; sequence analysis
-
Characterization of antioxidant activities and volatile profiles of pineapple beer during the brewing process
作者:Gong, Xiao;Yang, Qing;Chen, Ming;Tu, Jingxia;Gong, Xiao;Li, Bing;Ren, Fang
关键词:pineapple beer; brewing process; antioxidant activity; volatile organic compound
-
Genetic diversity analysis of grapevine rupestris stem pitting-associated virus from grapevine by colony PCR-SSCP and -RFLP
作者:Hu, Guo-Jun;Zhu, Hong-Juan;Zhang, Zun-Ping;Fan, Xu-Dong;Ren, Fang;Dong, Ya-Feng
关键词:grapevine; grapevine rupestris stem pitting-associated virus (GRSPaV); RFLP; SSCP; genetic diversity analysis