Heterogeneous Nuclear Ribonucleoprotein L Negatively Regulates Foot-and-Mouth Disease Virus Replication through Inhibition of Viral RNA Synthesis by Interacting with the Internal Ribosome Entry Site in the 5 ' Untranslated Region
文献类型: 外文期刊
第一作者: Sun, Chao
作者: Sun, Chao;Liu, Mengmeng;Chang, Jitao;Yang, Decheng;Zhao, Bo;Wang, Haiwei;Zhou, Guohui;Yu, Li;Weng, Changjiang
作者机构:
关键词: foot-and-mouth disease virus; internal ribosome entry site; heterogeneous nuclear ribonucleoprotein L; viral RNA synthesis; 3D(pol); virus-host interaction
期刊名称:JOURNAL OF VIROLOGY ( 影响因子:5.103; 五年影响因子:5.078 )
ISSN: 0022-538X
年卷期: 2020 年 94 卷 10 期
页码:
收录情况: SCI
摘要: Upon infection, the highly structured 5' untranslated region (5' UTR) of picornavirus is involved in viral protein translation and RNA synthesis. As a critical element in the 5' UTR, the internal ribosome entry site (IRES) binds to various cellular proteins to function in the processes of picornavirus replication. Foot-and-mouth disease virus (FMDV) is an important member in the family Picornaviridae, and its 5' UTR contains a functional IRES element. In this study, the cellular heterogeneous nu- clear ribonucleoprotein L (hnRNP was identified as an IRES-binding protein for FMDV by biotinylated RNA pulldown assays, mass spectrometry (MS) analysis, and determination of hnRNP L-IRES interaction regions. Further, we found that hnRNP L inhibited the growth of FMDV through binding to the viral IRES and that the inhibitory effect of hnRNP L on FMDV growth was not due to FMDV IRES-mediated translation, but to influence on viral RNA synthesis. Finally, hnRNP L was demonstrated to coimmunoprecipitate with RNA-dependent RNA polymerase (3D(pol)) in an FMDV RNA-dependent manner in the infected cells. Thus, our results suggest that hnRNP L, as a critical IRES-binding protein, negatively regulates FMDV replication by inhibiting viral RNA synthesis, possibly by remaining in the replication complex. IMPORTANCE Picornaviruses, as a large family of human and animal pathogens, cause a bewildering array of disease syndromes. Many host factors are implicated in the pathogenesis of these viruses, and some proteins interact with the viral IRES elements to affect function. Here, we report for the first time that cellular hnRNP L specifically interacts with the IRES of the picornavirus FMDV and negatively regulates FMDV replication through inhibiting viral RNA synthesis. Further, our results showed that hnRNP L coimmunoprecipitates with FMDV 3D(pol) in a viral RNA-dependent manner, suggesting that it may remain in the replication complex to function. The data presented here would facilitate further understanding of virus-host interactions and the pathogenesis of picornavirus infections.
分类号:
- 相关文献
作者其他论文 更多>>
-
Geographical origin identification of camellia oil based on fatty acid profiles combined with one-class classification
作者:Dou, Xinjing;Wang, Xuefang;Ma, Fei;Yu, Li;Mao, Jin;Jiang, Jun;Zhang, Liangxiao;Li, Peiwu;Zhang, Liangxiao;Mao, Jin;Zhang, Liangxiao;Li, Peiwu;Li, Peiwu
关键词:Camellia oil; Fatty acid profiles; Geographical origin traceability; One-class classification
-
Fe-N-C single-atom nanozyme-linked immunosorbent assay for quantitative detection of aflatoxin B1
作者:Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Huang, Xiaorong;Zhang, Zhaowei;Li, Peiwu;Li, Peiwu
关键词:Single -atom nanozyme; Nanozyme-linked immunosorbent assay; Aflatoxin B 1; Peanut; Peroxidase-like activity; Fe-N-C
-
Rapid detection of sesame oil multiple adulteration using a portable Raman spectrometer
作者:Li, Xue;Wang, Du;Ma, Fei;Yu, Li;Mao, Jin;Zhang, Wen;Jiang, Jun;Zhang, Liangxiao;Li, Peiwu;Zhang, Liangxiao;Mao, Jin;Zhang, Liangxiao;Li, Peiwu;Li, Peiwu;Zhang, Liangxiao
关键词:Sesame oil; Multiple adulteration; Raman spectroscopy; D -Optimal mixture design; Simplex theory of mixtures
-
Genome-Wide Identification of DUF668 Gene Family and Expression Analysis under Drought and Salt Stresses in Sweet Potato [Ipomoea batatas (L.) Lam]
作者:Liu, Enliang;Li, Zhiqiang;Luo, Zhengqian;Xu, Linli;Jin, Ping;Ji, Shun;Zhou, Guohui;Wang, Zhenyang;Zhou, Zhilin;Zhang, Hua
关键词:sweet potato; DUF668 gene family; bioinformatics; qRT-PCR
-
Genome-wide characterization of the SHORT INTER-NODES/STYLISH and Shi-Related Sequence family in Gossypium hirsutum and functional identification of GhSRS21 under salt stress
作者:Sun, Chendong;Zhang, Shuojun;Wang, Mei;Yu, Li;Gu, Qijuan
关键词:genome-wide characterization; SRS family; Gossypium hirsutum; salt stress; regulation of gene expression
-
Fe-N-C single-atom nanozyme for ultrasensitive, on-site and multiplex detection of mycotoxins using lateral flow immunoassay
作者:Cai, Xinfa;Ma, Fei;Jiang, Jun;Yang, Xianglong;Zhang, Zhaowei;Liang, Meijuan;Li, Peiwu;Yu, Li;Cai, Xinfa;Ma, Fei;Jiang, Jun;Yang, Xianglong;Zhang, Zhaowei;Liang, Meijuan;Li, Peiwu;Yu, Li;Cai, Xinfa;Ma, Fei;Jiang, Jun;Yang, Xianglong;Zhang, Zhaowei;Liang, Meijuan;Li, Peiwu;Yu, Li;Jiang, Jun;Li, Peiwu;Yu, Li;Jiang, Jun;Li, Peiwu;Yu, Li;Jian, Zelang
关键词:Single-atom nanozymes; Lateral flow immunoassay; Hydroxyl radicals; Catalytic mechanism; Mycotoxins
-
MBNet: A multi-branch network for detecting the appearance of Korla pears
作者:Li, Jia;Zhao, Bo;Wu, Jincan;Zhang, Shuaiyang;Wang, Feiyun;Lv, Chengxu
关键词:Multi-branch network; Integrated loss; Output re-judgement; End-to-end