Characteristics and roles of cytochrome b(5) in cytochrome P450-mediated oxidative reactions in Locusta migratoria
文献类型: 外文期刊
第一作者: Liu Jiao
作者: Liu Jiao;Zhang Xue-yao;Wu Hai-hua;Ma En-bo;Zhang Jian-zhe;Liu Jiao;Ma Wen;Zhu Wen-ya;Zhu, Kun-Yan
作者机构:
关键词: cytochrome b(5); cytochrome P450; cytochrome P450 reductase; Locusta migratoria; RNA interference
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2020 年 19 卷 6 期
页码:
收录情况: SCI
摘要: Cytochrome b(5) (Cyt-b(5)) is a small heme protein and known to be involved in a wide range of biochemical transformations, including cytochrome P450 monooxygenase (CYP)-mediated metabolism of endogenous and exogenous compounds. Studies on Cyt-b(5) are more concentrated in mammals, but are relatively rare in insects. The characteristics and function of Cyt-b(5) from Locusta migratoria have not been described yet. We sequenced the full-length cDNA sequence of Cyt-b(5) from L. migratoria (LmCyt-b(5)) by reverse transcription-PCR (RT-PCR) based on locust transcriptome database. The phylogenetic analysis showed that LmCyt-b(5) was closely related to the Cyt-b(5) from Blattodea. LmCyt-b(5) was highly expressed in ovary, Malpighian tubules, midgut, gastric caeca, and fat bodies. Silencing of LmCyt-b(5) had no effect on the susceptibility of L. migratoria to four different insecticides. Suppression of LmCyt-b(5) or silencing of both LmCyt-b(5) and LmCPR did not significantly change the total CYP activity toward the substrate 7-ethoxycoumarin (7-EC). However, coexpression of LmCYP6FD1 with LmCPR and LmCyt-b(5) together in Sf9 cells by using Bac-to-Bac baculovirus expression system significantly increased the catalytic activity of LmCYP6FD1 toward 7-EC as compared with the coexpression of LmCYP6FD1 with cytochrome P450 reductase (LmCPR) or LmCyt-b(5) separately. These results suggest that LmCyt-b(5) plays an important role in the catalytic reaction of LmCYP6FD1 toward 7-EC in our in vitro experiments. Further study is needed to clarify the role of LmCyt-b(5) in CYP-mediated catalytic reactions in L. migratoria.
分类号:
- 相关文献
作者其他论文 更多>>
-
Phylogeny of Limenitidinae Butterflies (Lepidoptera: Nymphalidae) Inferred from Mitochondrial Cytochrome Oxidase I Gene Sequences
作者:Zhang Min;Guo Ya-ping;Ma En-bo;Cao Tian-wen;Zhang Min;Zhong Yang
关键词:Nymphalidae;Limenitidinae;mtDNA;molecular phylogeny;cytochrome oxidase I gene