Integrating microneedle DNA extraction to hand-held microfluidic colorimetric LAMP chip system for meat adulteration detection

文献类型： 外文期刊

第一作者： Xiao, Bin

作者： Xiao, Bin;Zhao, Ruiming;Wang, Nan;Zhang, Juan;Sun, Xiaoyun;Huang, Fengchun;Chen, Ailiang

作者机构：

关键词： Microfluidic chip; Microneedle device; Colorimetric LAMP; Meat adulteration; Point-of-care testing

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 411 卷

页码：

收录情况： SCI

摘要： Most microfluidic-based "sample-in-result-out" systems suffer sophisticated microfluidic production processes, high-cost chips, and expensive instruments. They cannot be used in the meat market as well as farmer's markets in rural areas. Here, we developed a hand-held microfluidic chip system for on-site meat species qualitative authentication detection which integrated a simple microneedle DNA extraction and a visual loop-mediated isothermal amplification (LAMP). The chip can be used by easily pricking meat samples, simply hand-shaking the chip, and readily available isothermal heating instead of a complicated DNA extraction process and micro -fluidic control device. The system demonstrates high specificity and sensitivity for selected six species of meat samples and low to 1% simulated adulteration could be detected within 60 min. Besides, the whole cost was less than 1 dollar. The integrated hand-held microfluidic detection system offers a simple, fast, low-cost "sample-in -result-out" point-of-care device which could be extended to medical diagnosis and animal/plant disease identification.

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