Cytochrome P450 CYP6DB3 was involved in thiamethoxam and imidacloprid resistance in Bemisia tabaci Q (Hemiptera: Aleyrodidae)

文献类型： 外文期刊

第一作者： Wei, Xuegao

作者： Wei, Xuegao;Hu, Jinyu;Yin, Cheng;Xue, Hu;Zhang, Rong;Wang, Chao;Li, Junkai;Zhang, Youjun;Wei, Xuegao;Hu, Jinyu;Yang, Jing;Yin, Cheng;Du, Tianhua;Huang, Mingjiao;Fu, Buli;Gong, Peipan;Liang, Jinjin;Liu, Shaonan;Xue, Hu;He, Chao;Ji, Yao;Du, He;Zhang, Rong;Wang, Chao;Yang, Xin;Zhang, Youjun

作者机构：

关键词： Bemisia tabaci; Cytochrome P450; Neonicotinoid resistance; Metabolism

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2023 年 194 卷

页码：

收录情况： SCI

摘要： High level resistance for a variety of insecticides has emerged in Bemisia tabaci, a globally notorious insect. Neonicotinoid insecticides have been applied widely to control B. tabaci. Whether a differentially expressed gene CYP6DB3 discovered from transcriptome data of B. tabaci is involved in the resistance to neonicotinoid insecticides remains unclear. In the study, CYP6DB3 expression was significantly up-regulated in both thiamethoxam- and imidacloprid-resistant strains relative to the susceptive strains. We also found that CYP6DB3 expression was up-regulated after B. tabaci adults were exposed to thiamethoxam and imidacloprid. Moreover, knocking down CYP6DB3 expression via feeding corresponding dsRNA significantly reduced CYP6DB3 mRNA levels by 34.1%. Silencing CYP6DB3 expression increased the sensitivity of B. tabaci Q adults against both thiamethoxam and imidacloprid. Overexpression of CYP6DB3 gene reduced the toxicity of imidacloprid and thiamethoxam to transgenic D. melanogaster. In addition, metabolic studies showed that CYP6DB3 can metabolize 24.41% imidacloprid in vitro. Collectively, these results strongly support that CYP6DB3 plays an important role in the resistance of B. tabaci Q to imidacloprid and thiamethoxam. This work will facilitate a deeper insight into the part of cytochrome P450s in the evolution of insecticide resistance and provide a theoretical basis for the development of new integrated pest resistance management.

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