Experimental infection and comparative genomic analysis of a highly pathogenic PRRSV-HBR strain at different passage levels
文献类型: 外文期刊
第一作者: Wei, Yanwu
作者: Wei, Yanwu;Li, Shengbin;Huang, Liping;Tang, Qinghai;Liu, Jianbo;Liu, Dan;Wang, Yiping;Wu, Hongli;Liu, Changming
作者机构:
关键词: Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV);Virulence change;Genetic variation;Vaccine candidate
期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )
ISSN: 0378-1135
年卷期: 2013 年 166 卷 3-4 期
页码:
收录情况: SCI
摘要: A highly pathogenic strain of porcine reproductive and respiratory syndrome virus (PRRSV-HBR) was passaged on Marc-145 cells for 125 passages. In order to elucidate the change in virulence of PRRSV-HBR strain during the process of passage in vitro, swine infection experiment was performed with the viruses of low (F5 and F10) and high passage (F125). In addition, to identify the mutations related to the change in virulence of PRRSV-HBR strain, we compared and analyzed the genomic sequences of the F5, F10 and F125 of the strain. The virulence of F125 was significantly lower than that of F5 in the virus-infected pigs. In comparison with F5 and F125, there were 45 amino acids (aa) mutations and a deletion of 2 continuous aa by means of the virus genome sequence analysis. For these mutations, 33 aa (73.3%) occurred in the viral nonstructural proteins and the other 12 aa (26.7%) were contained in the viral structural proteins. Of the mutations, only 15 aa (33.3%) appeared in F10 and 30 aa (66.7%) occurred during passage from F10 to F125. The data showed that the latter 30 aa mutations were probably associated with attenuation of PRRSV-HBR strain, and that the change in virulence of the virus was determined by multiple alterations both in the structural and nonstructural genes. The virulence of PRRSV-HBR strain was remarkably attenuated after serial passages, and it can be used as vaccine candidate for control of the PRRS. (C) 2013 Elsevier B.V. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
A novel prebiotic enzymatic hydrolysate of citrus pectin during juice processing
作者:Lu, Xingmiao;Zhao, Chengying;Liu, Dan;Cui, Jiefen;Wang, Fengzhang;Zheng, Jinkai;Lu, Xingmiao;Zeng, Liang;Liu, Dan;Cui, Jiefen;Zheng, Jinkai;Hu, Mengxiao
关键词:Citrus juice; Pectin; Enzymatic hydrolysate; Chemical structure; Prebiotic
-
A novel all-natural (collagen plus pectin)/chitosan aqueous two-phase microcapsule with improved anthocyanin loading capacity
作者:Jiang, Zhenyang;Zhao, Shaojie;Zhao, Chengying;Zhang, Lin;Liu, Dan;Zheng, Jinkai;Jiang, Zhenyang;Zhao, Shaojie;Zhao, Chengying;Zhang, Lin;Liu, Dan;Zheng, Jinkai;Fan, Zhiying;Bao, Yuming
关键词:All-natural; Microcapsule; Aqueous two phase; Self-assembly; Loading ability
-
Metabolic Resistance of Sogatella furcifera (Hemiptera: Delphacidae) toward Pymetrozine Involves the Overexpression of CYP6FJ3
作者:Gong, Changwei;Wang, Xuegui;Gong, Changwei;Liu, Dan;Wang, Qiulin;Ma, Yanxin;Zhang, Shuirong;Awais, Muhammad;Pu, Jian;Yang, Jizhi;Wang, Xuegui;Zhan, Xiaoxu
关键词:Sogatella furcifera; Metabolic resistance; Rice; CYP6FJ3; Hydroxylate pymetrozine/pymetrozine
-
Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.]
作者:Cui, Yanjiao;Zhao, Zilong;Zhang, Jing;Li, Suying;Liu, Zhengli;Liu, Dan
关键词:foxtail millet; Chinese chestnut; spruce spider mite; crude protein content; transcriptome analysis
-
The structure-function relationships of pectins separated from three citrus parts: Flavedo, albedo, and pomace
作者:Zhang, Lin;Cui, Jiefen;Liu, Dan;Zhao, Cheng;Zheng, Jinkai;Zhang, Lin;Cui, Jiefen;Zhao, Shaojie;Zheng, Jinkai
关键词:Citrus; Pectin; Different parts; Chemical structure; Functional property
-
QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22
作者:Shawai, Rabiu Sani;Liu, Dan;Li, Lingli;Chen, Tiantian;Li, Ming;Cao, Shuanghe;Xia, Xianchun;Liu, Jindong;He, Zhonghu;Zhang, Yong;Shawai, Rabiu Sani;He, Zhonghu
关键词:Pre-harvest sprouting; Germination index; Quantitative trait loci; KASP marker; Triticum aestivum L
-
Impact of porcine circovirus type 2 on porcine epidemic diarrhea virus replication in the IPI-FX cell line depends on the order of infection
作者:Zhang, Hao;Shi, Hongyan;Wei, Yanwu;Shi, Da;Cao, Mengxiang;Liu, Jianbo;Liu, Jianhang;Li, Liang;Liu, Changming;Feng, Li;Huang, Liping
关键词:porcine circovirus type 2; porcine epidemic diarrhea virus; IPI-FX cells; IPEC-J2 cells; co-infection