Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor
文献类型: 外文期刊
第一作者: Wang, X. S.
作者: Wang, X. S.;Zhai, L. W.;Wang, C. D.;Yu, Y.;Ao, H.;Bai, L. J.;He, W. Y.
作者机构:
关键词: Porcine kidney epithelial cells;CD4;NF-kappa B;EMSA;DNA methylation
期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )
ISSN: 1676-5680
年卷期: 2014 年 13 卷 2 期
页码:
收录情况: SCI
摘要: The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFN alpha were significantly upregulated by PolyI:C, compared to the untreated PK15 cells, which shows that PolyI:C successfully mimics viral infection in PK15 cells. We also found that PolyI:C (10 mu g/ml) and/or Aza-CdR (5 mu M) significantly induces DNA demethylation of porcine CD4, promoting the binding of NF-kappa B to the CpG site on the CD4 promoter and activating expression of CD4. These data help clarify the regulatory mechanism of DNA methylation of the CD4 gene in non-immune cell response to virus replication. Further study is warranted to identify CD4 gene expression regulated by DNA methylation and live virus infection.
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