Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor
第一作者： Wang, X. S.
作者： Wang, X. S.;Zhai, L. W.;Wang, C. D.;Yu, Y.;Ao, H.;Bai, L. J.;He, W. Y.
关键词： Porcine kidney epithelial cells;CD4;NF-kappa B;EMSA;DNA methylation
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
年卷期： 2014 年 13 卷 2 期
摘要： The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFN alpha were significantly upregulated by PolyI:C, compared to the untreated PK15 cells, which shows that PolyI:C successfully mimics viral infection in PK15 cells. We also found that PolyI:C (10 mu g/ml) and/or Aza-CdR (5 mu M) significantly induces DNA demethylation of porcine CD4, promoting the binding of NF-kappa B to the CpG site on the CD4 promoter and activating expression of CD4. These data help clarify the regulatory mechanism of DNA methylation of the CD4 gene in non-immune cell response to virus replication. Further study is warranted to identify CD4 gene expression regulated by DNA methylation and live virus infection.
The Importance of Genetic Isolation in Preserving the Gene Pool of Apis cerana in Fanjingshan Nature Reserve
作者：Yu, Y.;Ran, Y.;Zhan, H.;Wan, W.;Wei, X.;Shi, L.;Li, M.
关键词：nature reserve; honey bee resource; protection; genetic differentiation; private haplotype
Genome-Wide Identification and Characterization of the E2F/DP Transcription Factor Family in Triticum aestivum L.
作者：Yu, Y.;Zhang, Y. R.;Song, T. Q.;Zhang, S. X.;Zhang, X. K.;Wang, X. L.;Chen, D. S.
关键词：Triticum aestivum L; E2F; DP; abiotic stress; subcellular localization; self-activation
IN VITRO PROTEIN DIGESTIBILITY OF BROWN RICE AFTER HIGH-PRESSURE FREEZE-THAW CYCLES AND GERMINATION-PARBOILING TREATMENTS
作者：Wang, H.;Zhu, S.;Li, T.;Yu, Y.;Wang, H.;Ramaswamy, H. S.;Ramaswamy, H. S.
关键词：Brown rice; Freeze-thaw cycles; Germination-parboiling; High-pressure; Protein in vitro digestibility
Sensitivity of in vitro digestible energy determined with computer-controlled simulated digestion system and its accuracy to predict dietary metabolizable energy for roosters
作者：Yu, Y.;Zhao, F.;Chen, J.;Zeng, S. L.;Zou, Y.;Liu, S. B.;Tan, H. Z.
关键词：diet; in vitro digestible energy; metabolizable energy; rooster; simulated digestion system
Selection and implementation of single nucleotide polymorphism markers for parentage analysis in crossbred cattle population
作者：Hu, L. R.;Li, D.;Wang, Y. C.;Zhou, L.;Yu, Y.;Zhang, Y.;Zhang, S. L.;Usman, T.;Li, D.;Chu, Q.;Usman, T.;Xie, Z. Q.;Hou, S. Y.;Liu, L.;Shi, W. H.
关键词：Holstein cattle; On-farm records; Parental errors; Simmental cattle; Single nucleotide polymorphism assays
Research Note: Effect of dietary cottonseed meal and soybean oil concentration on digesta passage time and amino acids digestibility in roosters
作者：Yu, Y.;Zhao, F.;Chen, J.;Wang, Y. M.;Zou, Y.;Liu, S. B.;Tan, H. Z.
关键词：amino acids; passage time; standardized ileal digestibility
A selection method of chickens with blue-eggshell and dwarf traits by molecular marker-assisted selection
作者：Cui, H. X.;Zheng, M. Q.;Wen, J.;Zhao, G. P.;Shen, Q. C.;Su, Y. C.;Cai, R. C.;Yu, Y.;Chen, Z. W.;Yang, X. R.
关键词：chicken; genetic aggregation; molecular marker-assisted breeding; dwarf; blue-eggshell