Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations
第一作者： Lu, J.
作者： Lu, J.;Zhou, D. H.;Chen, J.;Zhang, N. Z.;Wang, R. A.;Zhu, X. Q.;Lu, J.;Wang, R. A.;Weng, Y. B.
关键词： Toxoplasma gondii;Toxoplasmosis;Sequence variation;Heat shock protein 60;Phylogenetic analysis
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
年卷期： 2014 年 13 卷 3 期
摘要： The intracellular protozoan Toxoplasma gondii is one of the most successful parasites, with the ability to invade all warm-blooded animals, including humans. T. gondii heat shock protein 60 (TgHSP60) plays an important role in intracellular survival and in the differentiation of the parasite, and is also recognized as being associated with its virulence. In the present study, we examined sequence variation in the hsp60 coding region among five T. gondii isolates from different hosts and geographical regions, which were compared with the corresponding sequences of strains ME49, 76K, and GT1 available in the ToxoDB databases. The length of the T. gondii hsp60 sequence was 1728 bp for all strains, and the A+T content ranged from 41.96 to 42.13%. The sequence alignment of the 8 T. gondii strains identified 20 variable positions (0-1.44%) and showed 1.16% overall sequence variation, suggesting a relatively considerable sequence diversity. Phylogenetic analysis of hsp60 sequences using Bayesian inference and maximum parsimony differentiated the two major clonal lineage types into their respective clusters, and thus separated atypical strains from classical genotypes. The results of the present study suggested that the coding region of the hsp60 gene may represent a novel genetic marker for intraspecies phylogenetic analyses of T. gondii.
Sensitivity of in vitro digestible energy determined with computer-controlled simulated digestion system and its accuracy to predict dietary metabolizable energy for roosters
作者：Yu, Y.;Zhao, F.;Chen, J.;Zeng, S. L.;Zou, Y.;Liu, S. B.;Tan, H. Z.
关键词：diet; in vitro digestible energy; metabolizable energy; rooster; simulated digestion system
Research Note: Effect of dietary cottonseed meal and soybean oil concentration on digesta passage time and amino acids digestibility in roosters
作者：Yu, Y.;Zhao, F.;Chen, J.;Wang, Y. M.;Zou, Y.;Liu, S. B.;Tan, H. Z.
关键词：amino acids; passage time; standardized ileal digestibility
Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency
作者：Lu, X.;Liu, S.;Zhi, S.;Chen, J.;Ye, G.;Liu, S.;Ye, G.;Zhi, S.;Ye, G.
关键词：Differentially expressed genes; Oryza sativa L; roots; transcriptome; Zn deficiency
Effects of different combinations of N, P and K at different time interval on vegetative, reproductive, yield and quality traits of mango (Mangifera Indica. L) cv. Dusehri
作者：Azam, M.;Qadri, R.;Khan, A. S.;Anwar, R.;Ghani, M. A.;Aslam, A.;Khan, M., I;Ejaz, S.;Hussain, Z.;Iqbal, M. A.;Chen, J.
关键词：NPK fertilizers; vegetative and reproductive growth; fruit quality; yield; Dusehri
Genome-wide association studies for mottled eggs in chickens using a high-density single-nucleotide polymorphism array
作者：Qu, L.;Shen, M. M.;Dou, T. C.;Ma, M.;Lu, J.;Wang, X. G.;Guo, J.;Hu, Y. P.;Li, Y. F.;Wang, K. H.;Shen, M. M.
关键词：Egg quality; Genomic dissection; Layer hen; Quantitative trait loci; Variance explained by single-nucleotide; polymorphism
Characterizing the impact of high temperature during grain filling on phytohormone levels, enzyme activity and metabolic profiles of an early indica rice variety
作者：Shao, C.;Qiu, C.;Qian, Y.;Chen, J.;Guan, X.;Xie, J.;Liu, G.;Peng, C.;Shao, C.;Wang, Y.;Ouyang, Z.;Zhang, P.;Shen, L.
关键词：high temperature stress (HTS); indica rice; sucrose-starch metabolism; phytohormone homeostasis; metabolic profiling
Transcriptome analysis of sheep follicular development during prerecruitment, dominant, and mature stages after FSH superstimulation
作者：Yao, Y. C.;Song, X. T.;Zhai, Y. F.;Liu, S.;Lu, J.;Xu, X.;Zhang, J. N.;Huang, H.;Yuan, H.;Yao, Y. C.;Song, X. T.;Zhai, Y. F.;Liu, S.;Lu, J.;Xu, X.;Zhang, J. N.;Huang, H.;Yuan, H.;Qi, M. Y.;Liu, Y. F.;Liu, G. S.
关键词：RNA-seq; Ovarian steroidogenesis; Sheep; Follicular development; FSH superstimulation