Pilot-scale membrane fractionation of ACE inhibitory and antioxidative peptides from ultrasound pretreated milk protein concentrate hydrolysates

文献类型： 外文期刊

第一作者： Uluko, Hankie

作者： Uluko, Hankie;Zhang, Shuwen;Liu, Lu;Li, Hongjuan;Cui, Wenming;Xue, Haixiao;Zhao, Lili;Lu, Jing;Lv, Jiaping;Uluko, Hankie;Sun, Yanjun

作者机构：

关键词： Milk protein concentrate;Ultrasound;Hydrolysis;Angiotensin converting enzyme;Antioxidative;Membrane filtration

期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：4.451； 五年影响因子：4.907 ）

ISSN： 1756-4646

年卷期： 2014 年 7 卷

页码：

收录情况： SCI

摘要： A batch mode, pilot-scale process, involving ultrasound pretreatment (USP), enzymolysis, membrane filtration and drying was investigated for the production of angiotensin converting enzyme (ACE) inhibitory and antioxidative peptides from milk protein concentrate (MPC). The MPC solutions (2% or 5% protein) were hydrolyzed for 2 h with Neutrase at enzyme-to-substrate ratio of 1:50 (w/w) in 50 L batches after USP for 4 min at 800 W Ultrafiltration (8 and 3.5 kDa membranes) and nanofiltration (0.2 kDa membrane) were done to produce 4 fractions (F1, >8 kDa; F2, 3.5-8 kDa; F3, 0.2-3.5 kDa; and F4, <0.2 kDa). Analyses showed that large amounts of ACE inhibitory peptides were recovered by the 3.5 kDa membrane and concentrated by nanofiltration making F3 ideal for ACE inhibition (IC50 = 0.096 +/- 0.010 mg mL(-1)) while antioxidative peptides were abundant in F2 with bioactivity not affected by drying method. Initial substrate concentration had no significant effect (P <0.05) on bioactivity. Furthermore, permeation fluxes were high when a low protein concentration was used. Ultrafiltration achieved peptide rejections rates in the range of 24.0-33.7% and reached 89.6% during nanofiltration. The results indicate that membrane filtration can separate ACE inhibitory and antioxidative peptides in complex protein hydrolysates. (C) 2014 Elsevier Ltd. All rights reserved.

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