Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs
文献类型: 外文期刊
第一作者: Feng, Hui
作者: Feng, Hui;Liu, Tianyi;Yousuf, Salsabeel;Zhang, Xiuxiu;Huang, Wanlong;Li, Ai;Xie, Lingli;Miao, Xiangyang
作者机构:
关键词: lncRNA; co-expression; ceRNA network; intramuscular fat; adipogenesis
期刊名称:FRONTIERS IN ENDOCRINOLOGY ( 影响因子:5.2; 五年影响因子:5.9 )
ISSN: 1664-2392
年卷期: 2023 年 13 卷
页码:
收录情况: SCI
摘要: BackgroundLong non-coding RNAs (lncRNAs) regulate adipocyte differentiation and metabolism, However, their function on subcutaneous and intramuscular adipose tissues in pigs is unclear. Intramuscular fat (IMF) is an important indicator for evaluating meat quality. Breeds with high IMF content are often accompanied by high subcutaneous fat (SCF), which severely affects the meat rate of pigs. It is of great significance for porcine breeding to study the mechanism of lncRNA related to adipogenesis and lipid metabolism. MethodsWe identified differentially expressed lncRNAs, miRNAs and mRNAs in subcutaneous and intramuscular adipose tissues in three female Laiwu pigs by deep RNA-sequencing(|log2foldchange|>= 1, P_value <= 0.05). The gene expression profiles of IMF and SCF in Laiwu pigs were comparatively analyzed by Bioinformatics methods to identify key lncRNAs, miRNAs, and mRNAs associated with lipid metabolism and adipogenesis. ResultsA total of 1209 lncRNAs (DElncRNAs), 286 miRNAs (DEmiRNAs), and 1597 mRNAs (DEgenes) were differentially expressed between two types of adipose. Among them, 17 DElncRNAs and 103 target genes play a role in the co-expression network, as well as 59 DElncRNAs, 44 DEmiRNAs, and 88 DEgenes involved in ceRNA network. In GO(Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis of DElncRNAs their target genes involved in many adipogenesis and lipid metabolism biological processes and signaling pathways, such as PPAR signaling pathway, Wnt signaling pathway, MAPK signaling pathway. ConclusionsBy constructing co-expression and ceRNAs network we found that Wnt signaling pathway play a critical regulatory role in intramuscular adipogenesis and lipid accumulation in Laiwu pigs. TCONS_00006525, TCONS_00046551 and TCONS_00000528 may target WNT5A, WNT10B and FDZ3 in co-expression network, TCONS_00026517 and other lncRNAs regulate the expression of PPARG, RXRG and SCD in ceRNA network, and were involved in Wnt signaling pathway. This study provides a theoretical basis for further understanding the post-transcriptional regulation mechanism of meat quality formation, predicting and treating diseases caused by ectopic fat.
分类号:
- 相关文献
作者其他论文 更多>>
-
Integrative Analysis of miRNAs Involved in Fat Deposition in Different Pig Breeds
作者:Zhang, Xiuxiu;Huang, Wanlong;Guo, Yuntao;Miao, Xiangyang
关键词:pig; RNA-seq; miRNAs; adipose tissue; PPAR
-
Impact of FecB Mutation on Ovarian DNA Methylome in Small-Tail Han Sheep
作者:Xie, Lingli;Miao, Xiangyang;Luo, Qingmiao;Zhao, Huijing;Qin, Xiaoyu
关键词:sheep; epigenetics; ovary; methylation; fecundity
-
Star polymer soil delivery nanoplatform for applying biological agents in the field to control plant rhizosphere diseases
作者:Su, Chenyu;Li, Yiting;Liu, Shanshan;Feng, Hui;Wang, Jie;Yan, Shuo
关键词:Plant rhizosphere diseases; Naringenin; Star polymer; Soil delivery nanoplatform
-
Functional analysis of differentially expressed circular RNAs in sheep subcutaneous fat
作者:Liu, Tian-yi;Feng, Hui;Yousuf, Salsabeel;Xie, Ling-li;Miao, Xiang-yang
关键词:Sheep; CircRNA; Subcutaneous fat; Fat deposition; Lipid metabolism
-
Comparative analysis of alternative splicing events in skeletal muscle of different sheep
作者:Miao, Xiangyang;Luo, Qingmiao;Zhao, Huijing;Qin, Xiaoyu
关键词:Sheep; Muscle; Alternative splicing; RNA-seq; mRNAs
-
Volatile Organic Compounds from Pythium oligandrum Play a Role in Its Parasitism on Plant-Pathogenic Pythium myriotylum
作者:Sheikh, Taha Majid Mahmood;Zhou, Dongmei;Wang, Nan;Chen, Siqiao;Zhao, Yishen;Wen, Xian;Feng, Hui;Wang, Xiaoyu;Zhang, Jinfeng;Deng, Sheng;Wei, Lihui;Daly, Paul;Haider, Muhammad Salman;Hussain, Sarfraz;Chen, Siqiao;Zhao, Yishen;Wen, Xian;Wang, Lunji;Raza, Waseem;Wei, Lihui
关键词:P; oligandrum; myriotylum; microbe-microbe interaction; RNAseq; GC-MS; volatile organic compounds
-
Nephroprotective Effect of Cinnamon cassia and Azadirachta indica on Titanium Dioxide Nanoparticles
作者:Shabbir, Samina;Miao, Xiangyang;Shabbir, Samina;Boruah, Prerona;Kulyar, Muhammad Fakhar-e-Alam;Bhutta, Zeeshan Ahmad;Nawaz, Mohsin;Ashar, Ambreen;Mahfooz, Ashar;Khan, Muhammad Saleem;Jabeen, Farhat;Mengal, Kifayatullah
关键词:Nanoparticles; nephrotoxicity; Cinnamomum cassia; Azadirachta indica; TiO2; histology; nanotechnology