Recombinant Expression of a Novel Fungal Immunomodulatory Protein with Human Tumor Cell Antiproliferative Activity from Nectria haematococca
文献类型: 外文期刊
第一作者: Li, Shuying
作者: Li, Shuying;Nie, Ying;Ding, Yang;Tang, Xuanming;Shi, Lijun
作者机构:
关键词: antitumor;fungal immunomodulatory protein (FIP);hemagglutination;interleukin-2 (IL-2);Nectria haematococca;proliferation
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2014 年 15 卷 10 期
页码:
收录情况: SCI
摘要: To our best knowledge, all of the fungal immunomodulatory proteins (FIPs) have been successfully extracted and identified in Basidomycetes, with only the exception of FIP from ascomycete Nectria haematococca (FIP-nha) discovered through homology alignment most recently. In this work, a gene encoding FIP-nha was synthesized and recombinantly expressed in an Escherichia coli expression system. SDS-PAGE and MALDI-MS analyses of recombinant FIP-nha (rFIP-nha) indicated that the gene was successfully expressed. The yield of the bioactive FIP-nha protein was 42.7 mg/L. In vitro assays of biological activity indicated that the rFIP-nha caused hemagglutination of human and rabbit red blood cells, significantly stimulated mouse spleen lymphocyte proliferation, and enhanced expression of interleukin-2 (IL-2) released from mouse splenocytes, revealing a strong antitumor effect against HL60, HepG2 and MGC823. Through this work, we constructed a rapid and efficient method of FIP production, and suggested that FIP-nha is a valuable candidate for use in future medical care and pharmaceutical products.
分类号:
- 相关文献
作者其他论文 更多>>
-
Effects of ultra-high pressure, thermal pasteurization, and ultra-high temperature sterilization on color and nutritional components of freshly-squeezed lettuce juice
作者:Zhang, Jiadi;Cheng, Jinxiao;Li, Zhixuan;Zhang, Xudong;Tang, Xuanming;Pan, Yanfang;Zhang, Jiadi;Cheng, Jinxiao;Weng, Mengting
关键词:Freshly -squeezed lettuce juice; Sterilization; Ultra -high Pressure; Color; Nutritional components
-
Metabolomics and lipidomics profiles related to intramuscular fat content and flavor precursors between Laiwu and Yorkshire pigs
作者:Hou, Xinhua;Zhang, Run;Yang, Man;Niu, Naiqi;Wu, Junchao;Shu, Ze;Zhang, Pengfei;Shi, Lijun;Zhao, Fuping;Wang, Ligang;Wang, Lixian;Zhang, Longchao
关键词:Pig; IMF; Meat flavor; Metabolomics; Lipidomics
-
The structural and functional properties of soybean protein-polyglutamic acid complex effected the stability of W/O/W emulsion encapsulated Nattokinase
作者:Li, Danfeng;Hu, Miao;Hou, Lizhen;Gao, Yaxin;Tian, Zhiliang;Wen, Wei;Fan, Bei;Li, Shuying;Wang, Fengzhong;Wang, Fengzhong;Wang, Fengzhong
关键词:Nattokinase; Soybean protein; Polyglutamic acid; Non-covalent complex; W; O; W emulsion
-
Plant Architecture Influences the Population Transpiration and Canopy Temperature in Winter Wheat Genotypes
作者:Huang, Guirong;Zhang, Xinying;Wang, Zhenzhao;Li, Yuzhong;Liu, Xiaoying;Guo, Rui;Gu, Fengxue;Liu, Enke;Zhong, Xiuli;Mei, Xurong;Huang, Guirong;Zhang, Xinying;Li, Shuying
关键词:winter wheat; plant architecture; canopy temperature depression; population-scale transpiration; evaporation
-
Dynamic variation of nutrient absorption, metabolomic and transcriptomic indexes of soybean (Glycine max) seedlings under phosphorus deficiency
作者:Li, Mingxia;Zhou, Ji;Liu, Qi;Mao, Lili;Li, Haoru;Guo, Rui;Li, Shuying
关键词:Glycine max; metabolomic; phosphorus deficiency; roots; transcriptomic
-
Integrated analysis of transcriptome and metabolome revealed biological basis of sows from estrus to lactation
作者:Shi, Lijun;Li, Huihui;Huang, Xiaoyu;Shu, Ze;Li, Jingna;Wang, Ligang;Yan, Hua;Wang, Lixian
关键词:
-
Genetic parameters estimation and genome molecular marker identification for gestation length in pigs
作者:Shi, Lijun;Li, Huihui;Wang, Lixian
关键词:pig gestation length; genetic parameter estimation; GWAS; Fst; genetic molecular marker