Global and quantitative proteomic analysis of dogs infected by avian-like H3N2 canine influenza virus

文献类型： 外文期刊

第一作者： Su, Shuo

作者： Su, Shuo;Hong, Malin;Zhou, Pei;Lu, Gang;Zhang, Guihong;Li, Shoujun;Su, Shuo;Hong, Malin;Zhou, Pei;Lu, Gang;Li, Shoujun;Tian, Jin;Zhu, Huachen;Zhu, Huachen;Lai, Alexander

作者机构：

关键词： canine influenza virus;H3N2;global proteomic analysis;tandem mass spectrometry

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2015 年 6 卷

页码：

收录情况： SCI

摘要： Canine influenza virus A (H3N2) is a newly emerged etiological agent for respiratory infections in dogs. The mechanism of interspecies transmission from avian to canine species and the development of diseases in this new host remain to be explored. To investigate this, we conducted a differential proteomics study in 2-month-old beagles inoculated intranasally with 10(6) TCID50 of A/canine/Guangdong/01/2006 (H3N2) virus. Lung sections excised at 12 h post-inoculation (hpi), 4 days, and 7 days post-inoculation (dpi) were processed for global and quantitative analysis of differentially expressed proteins. A total of 17,796 proteins were identified at different time points. About 1.6% was differentially expressed between normal and infected samples. Of these, 23, 27, and 136 polypeptides were up-regulated, and 14, 18, and 123 polypeptides were down-regulated, at 12 hpi, 4 dpi, and 7 dpi, respectively. Vann diagram analysis indicated that 17 proteins were up-regulated and one was down-regulated at all three time points. Selected proteins were validated by real-time PCR and by Western blot. Our results show that apoptosis and cytoskeleton-associated proteins expression was suppressed, whereas interferon-induced proteins plus other innate immunity proteins were induced after the infection. Understanding of the interactions between virus and the host will provide insights into the basis of interspecies transmission, adaptation, and virus pathogenicity.

分类号：

  • 相关文献

[1]Characterization of an H3N2 canine influenza virus isolated from Tibetan mastiffs in China. Teng, Qiaoyang,Xu, Dawei,Zhou, Jiewen,Dai, Xiaoguang,Chen, Zhaoguo,Li, Zejun,Zhang, Xu. 2013

[2]Seroprevalence of avian origin H3N2 canine influenza virus infection in pet dogs in Shenzhen, China. Zhao, Fu-Rong,Li, Shou-Jun,Qi, Wen-Bao,Jiao, Pei-Rong,Liao, Ming,Zhang, Gui-Hong,Zhao, Fu-Rong,Zhou, Dong-Hui,Chen, Ning,Zhang, Yan-Zhong,Tong, Guang-Zhi. 2011

[3]Development of DIVA (differentiation of infected from vaccinated animals) vaccines utilizing heterologous NA and NS1 protein strategies for the control of triple reassortant H3N2 influenza in turkeys. Wang, Leyi,Qin, Zhuoming,Saifa, Yehia M.,Lee, Chang-Won,Wang, Leyi,Saifa, Yehia M.,Lee, Chang-Won,Qin, Zhuoming,Pantin-Jackwood, Mary,Faulkner, Olivia,Suarez, David L.,Garcia, Maricarmen,Lupiani, Blanca,Reddy, Sanjay M.. 2011

[4]The effects of H3N2 swine influenza virus infection on TLRs and RLRs signaling pathways in porcine alveolar macrophages. Zhang, Jinqiu,Hou, Jibo,Zhang, Jinqiu,Miao, Jinfeng,Lu, Chengping. 2015

[5]A novel M2e-multiple antigenic peptide providing heterologous protection in mice. Wen, Feng,Ma, Ji-Hong,Yu, Hai,Yang, Fu-Ru,Huang, Meng,Zhou, Yan-Jun,Li, Ze-Jun,Wang, Xiu-Hui,Li, Guo-Xin,Jiang, Yi-Feng,Tong, Wu,Tong, Guang-Zhi.

[6]High genetic and antigenic similarity between a swine H3N2 influenza A virus and a prior human influenza vaccine virus: A possible immune pressure-driven cross-species transmission. Pan, Chungen,Jiang, Shibo,Wang, Guiping,Liao, Ming,Zhang, Gui-Hong. 2009

[7]Multiple amino acid substitutions involved in the virulence enhancement of an H3N2 avian influenza A virus isolated from wild waterfowl in mice. Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Gao, Yuwei,Xia, Xianzhu,Gao, Yuwei,Xia, Xianzhu,Cheng, Kaihui,Zhao, Chuqi.

[8]Comprehensive identification of novel proteins and N-glycosylation sites in royal jelly. Zhang, Lan,Han, Bin,Li, Rongli,Lu, Xiaoshan,Fang, Yu,Feng, Mao,Li, Jianke,Zhang, Lan,Lu, Xiaoshan,Nie, Aiying,Guo, Lihai. 2014

[9]Detection of 13 mycotoxins in feed using modified QuEChERS with dispersive magnetic materials and UHPLC-MS/MS. Qian, Mingrong,Yang, Hua,Wang, Jianmei,Ji, Xiaofeng,Xu, Jie,Li, Zuguang,Liu, Yanping,Wu, Huizhen. 2018

[10]Performance comparison of dispersive solid-phase extraction and multiplug filtration cleanup methods for the determination of tefuryltrione in plant and environmental samples using UHPLC-MS/MS. Yu, Yang,Ji, Mingshan,Tao, Yan,Dong, Fengshou,Xu, Jun,Liu, Xingang,Wu, Xiaohu,Zheng, Yongquan. 2017

[11]Comparison of different cleanup procedures for oil crops based on the development of a trace analytical method for the determination of pyraclostrobin and epoxiconazole. Dong, Fengshou,Xu, Jun,Liu, Xingang,Cheng, Youpu,Chen, Zenglong,Liu, Na,Chen, Xixi,Tao, Yan,Zheng, Yongquan.

[12]Determination of five sulfonylurea herbicides in environmental waters and soil by ultra high performance liquid chromatography with tandem mass spectrometry after extraction using graphene. Li, Cheng,Lu, Anxiang,Wang, Jihua,Ping, Hua,Luan, Yunxia,Chen, Jiayi,Lu, Anxiang,Li, Jie,Ha, Xuejiao.

[13]Determination of neonicotinoid insecticides residues in eels using subcritical water extraction and ultra-performance liquid chromatography-tandem mass spectrometry. Xiao, Zhiming,Yang, Yunxia,Li, Yang,Fan, Xia,Ding, Shuangying.

[14]Magnetic molecularly imprinted polymers for the determination of beta-agonist residues in milk by ultra high performance liquid chromatography with tandem mass spectrometry. Liu, Hongcheng,Lin, Xin,Lin, Tao,Luo, Yinglan,Li, Qiwan,Liu, Hongcheng,Lin, Xin,Lin, Tao,Luo, Yinglan,Li, Qiwan,Liu, Hongcheng,Lin, Xin,Lin, Tao,Luo, Yinglan,Li, Qiwan,Zhang, Yulong.

[15]Fragmentation of protonated 2-(2-phenylethyl)chromones from agarwood: the diagnostic role of ion/neutral complexes as reactive intermediates. Yang, Delan,Mei, Wenli,Xia, Bing,Jiang, Yan,Kuck, Dietmar.

[16]Towards posttranslational modification proteome of royal jelly. Zhang, Lan,Fang, Yu,Li, Rongli,Feng, Mao,Han, Bin,Zhou, Tiane,Li, Jianke.

[17]Simultaneous determination of florfenicol with its metabolite based on modified quick, easy, cheap, effective, rugged, and safe sample pretreatment and evaluation of their degradation behavior in agricultural soils. Xu, Mingfei,Qian, Mingrong,Zhang, Hu,Wang, Jianmei,Wu, Huizhen,Ma, Junwei.

[18]Comparative proteomics of leaves found at different stem positions of maize seedlings. Chen, Yi-Bo,Zhao, Biligen-Gaowa,Wang, Bai-Chen,Wang, Dan,Wang, Xu-Chu,Ge, Xuan-Liang.

[19]Simultaneous determination of Ltx and Ltxd in cured meat products by LC/MS/MS. Song, Hui,Wu, Haihong,Geng, Zhiming,Sun, Chong,Ren, Shuang,Wang, Daoying,Zhang, Muhan,Liu, Fang,Xu, Weimin,Song, Hui,Ren, Shuang,Xu, Weimin.

[20]Simultaneous Determination of 13-HODE, 9,10-DHODE, and 9,10,13-THODE in Cured Meat Products by LC-MS/MS. Song, Hui,Wu, Haihong,Geng, Zhiming,Sun, Chong,Ren, Shuang,Wang, Daoying,Zhang, Muhan,Liu, Fang,Xu, Weimin,Song, Hui,Ren, Shuang,Xu, Weimin.

作者其他论文 更多>>

微信小程序