Characterization of a Novel C-type Lectin against OsHV-1 infection in Scapharca broughtonii

文献类型： 外文期刊

第一作者： Wang, D.

作者： Wang, D.;Zhou, H.;Wang, D.;Huang, B.;Yu, J.;Bai, C.;Li, C.;Wang, C.;Xin, L.;Wang, D.;Huang, B.;Yu, J.;Bai, C.;Li, C.;Wang, C.;Xin, L.;Yu, J.

作者机构：

关键词： Scapharca broughtonii; C-type lectin; PAMP binding; OsHV-1 infection

期刊名称：ISJ-INVERTEBRATE SURVIVAL JOURNAL （ 影响因子：0.917； 五年影响因子：1.263 ）

ISSN： 1824-307X

年卷期： 2022 年 19 卷

页码：

收录情况： SCI

摘要： As important pattern recognition receptors (PRRs), most C-type lectins (CTLs) are a class of Ca2+-dependent carbohydrate-binding proteins that are found to be involved in non-self-recognition and antiviral process. In this study, a new CTL, named SbCTL, was identified from ark clams, Scapharca broughtonii. The amino acid sequence of SbCTL consisted of a predicted CRD (carbohydrate recognition domain) structural domain (including 102 amino acid residues). The amino acid sequence of SbCTL shared 28 % - 39 % similarity with other CTLs. There were two potential Ca2+ binding sites in SbCTL. The expression of SbCTL mRNA was detected in all selected tissues, with the highest expression in the gills. Expression of SbCTL mRNA increased significantly (p < 0.05) and total vibrio content increased significantly (p < 0.05) in ark clam infected with OsHV-1 at 72 h post-infection. The binding activities of recombinant SbCTL (rSbCTL) to various PAMPs with or without Ca2+ were analyzed by ELISA, rSbCTL showed especially high binding activity to LPS in a Ca2+-independent manner. rSbCTL also functioned on sheltering ark clams from OsHV-1 infection in vivo, there were less mortality occurred in the rSbCTL treated group than the control. In all, it suggests that SbCTL, could served a critical role in the immune response against intruders in ark clams.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序