Identification of three wheat near isogenic lines originated from CB037 on tissue culture and transformation capacities

文献类型： 外文期刊

第一作者： Liu, Huiyun

作者： Liu, Huiyun;Ma, Haili;Zhang, Wei;Wang, Wanqing;Wang, Ke;Lin, Zhishan;Ye, Xingguo;Liu, Huiyun;Ma, Haili;Wu, Jiajie

作者机构：

关键词： Wheat; Near isogenic lines; Disease resistance; Tissue culture; Genetic transformation

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.726； 五年影响因子：2.859 ）

ISSN： 0167-6857

年卷期： 2023 年 152 卷 1 期

页码：

收录情况： SCI

摘要： Key message CB037B was high resistant to PM and stripe rust. It had high regeneration ability in anther, immature, and mature embryo culture, and could be used in wheat genetic transformation for generating transgenic plants. To meet the increasing demand for wheat grains, it is essential to continuously improve wheat by commercial and advanced technologies. Wheat line CB037 has ideal agronomic traits, good bread quality, resistance to powdery mildew (PM), and high regeneration ability, and therefore it has been widely used in hybridization breeding and genetic transformation. Three near isogenic lines (NILs) from CB037 were identified in a previous study based on glutenin composition. In this study, the three NILs of CB037A, CB037B, and CB037C were investigated comparatively for agronomic traits, disease resistance to PM and stripe rust, and tissue culture and transformation efficiency. Results indicated that these NILs were highly resistant to PM, and CB037B was not only highly resistant to stripe rust but also had significantly higher regeneration ability than CB037A and CB037C in anther culture, immature and mature embryo culture. Particularly, positively transgenic wheat plants were obtained only from CB037B. Thereby CB037B carrying two types of translocations of 6AL center dot 6V#2S and 1BL center dot 1RS was identified to be a desirable material for wheat genetic transformation, which was of great significance for improving the transformation efficiency and accelerating the application of genetic engineering breeding technology in wheat.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序