Effects of dietary reduced glutathione on growth performance, non-specific immunity, antioxidant capacity and expression levels of IGF-I and HSP70 mRNA of grass carp (Ctenopharyngodon idella)

文献类型： 外文期刊

第一作者： Ming, Jian-Hua

作者： Ming, Jian-Hua;Ye, Jin-Yun;Zhang, Yi-Xiang;Ming, Jian-Hua;Xu, Pao;Xie, Jun

作者机构：

关键词： Ctenopharyngodon idella;Reduced glutathione (GSH);Non-specific immunity;Antioxidant capacity;Gene expression

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： This study was conducted to evaluate the effects of dietary reduced glutathione (GSH) on growth performance, non-specific immunity, antioxidant capacity and related gene expression of grass carp (Ctenopharyngodon idella). Six hundred and thirty juvenile grass carp with initial body weight of 5.10 +/- 0.09 g were randomly distributed into six groups with three replicates each, fed six diets containing graded levels of GSH (14.26, 112.41, 210.24, 407.45, 602.85 and 798.32 mg/kg diet) for 60 days. At the end of the feeding trail, ten fish per tank were challenged with Aeromonas hydrophila and the cumulative mortalities were recorded for 7 days. The results showed that the weight gain (WG), specific growth rate (SGR) and survival rate of grass carp, serum total protein (TP), lysozyme, alkaline phosphatase (ALP), and complement 3 (C3) levels, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in the liver, and liver total antioxidant capacity (T-AOC) and GSH contents, and expression levels of IGF-I and HSP70 mRNA significantly increased, while feed conversion rate (FCR), liver glutathione reductase (GR) activity and malondialdehyde (MDA) content significantly decreased with dietary GSH levels up to 407.45 mg/kg diet (P < 0.05), whereas dietary excess GSH had a negative effect on the above parameters. However, activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and GSH content in serum were not significantly affected by dietary GSH contents among all groups (P > 0.05). The challenge experiment showed that the cumulative mortality significantly decreased with dietary GSH contents up to 407.45 mg/kg diet (P < 0.05), and then increased. Thus, the diet supplemented with a certain amount of GSH could promote the growth of juvenile grass carp, improve survival rate and lower FCR, increase nonspecific immunity, antioxidant capacity, and expression levels of IGF-I and HSP70 mRNA, and enhance the resistance to pathogen infection. However, dietary excess GSH had negative effect on fish. Based on second-degree polynomial regression analysis of weight gain, the optimum requirement of dietary GSH in juvenile grass carp is 381 mg/kg diet. (C) 2014 Elsevier B. V. All rights reserved.

分类号： S9

  • 相关文献

[1]Dietary supplementation with carnosine improves antioxidant capacity and meat quality of finishing pigs. Ma, X. Y.,Jiang, Z. Y.,Lin, Y. C.,Zheng, C. T.,Zhou, G. L.,Ma, X. Y.,Jiang, Z. Y.,Lin, Y. C..

[2]Modulation of exogenous glutathione in antioxidant defense system against Cd stress in the two barley genotypes differing in Cd tolerance. Chen, Fei,Wang, Fang,Wu, Feibo,Mao, Weihua,Zhang, Guoping,Chen, Fei,Zhou, Meixue.

[3]Effects of Dietary Emodin Supplementation on Growth Performance, Non-Specific Immune Responses, and Disease Resistance to Aeromonas hydrophila in Juvenile Wuchang Bream (Megalobrama amblycephala). Zhang, Yuan-yuan,Liu, Bo,Ge, Xian-ping,Xie, Jun,Cui, Yan-ting,Zhang, Yuan-yuan,Liu, Bo,Ge, Xian-ping,Xie, Jun,Ren, Ming-chun,Zhou, Qun-lan,Sun, Sheng-ming,Cui, Yan-ting,Chen, Ru-li,Pan, Liang-kun,Zhang, Yuan-yuan,Liu, Wen-bin. 2014

[4]Effect of bioactive peptides (BPs) on the development of Pacific white shrimp (Litopenaeus vannamei Boone, 1931). Wang Guangjun,Yu Ermeng,Li Zhifei,Yu Deguang,Wang Haiying,Gong Wangbao. 2016

[5]Effects of dietary beta-1,3-glucan, chitosan or raffinose on the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Lin, Shimei,Pan, Yu,Luo, Li,Luo, Lin. 2011

[6]The influence of various feeding patterns of emodin on growth, non-specific immune responses, and disease resistance to Aeromonas hydrophila in juvenile Wuchang bream (Megalobrama amblycephala). Zhang, Yuan-yuan,Liu, Bo,Ge, Xian-ping,Liu, Wen-bin,Xie, Jun,Cui, Yan-ting,Xia, Si-lei,Zhang, Yuan-yuan,Liu, Bo,Ge, Xian-ping,Xie, Jun,Ren, Mingchun,Cui, Yan-ting,Xia, Si-lei,Chen, Ruli,Zhou, Qunlan,Pan, Liangkun,Zhang, Yuan-yuan,Liu, Wen-bin,Yu, Yebing. 2014

[7]Effects of dietary Saccharomyces cerevisiae fermentation product (DVAQUA (R)) on growth performance, intestinal autochthonous bacterial community and non-specific immunity of hybrid tilapia (Oreochromis niloticus female xO. aureus male) cultured in cages. He, Suxu,Zhou, Zhigang,Liu, Yuchun,Shi, Pengjun,Yao, Bin,Ringo, Einar,Ringo, Einar,Yoon, Ilkyu. 2009

[8]Effect of dietary copper on the growth performance, non-specific immunity and resistance to Aeromonas hydrophila of juvenile Chinese mitten crab, Eriocheir sinensis. Sun, Shengming,Yu, Na,Jiang, Haibo,Chen, Liqiao,Qin, Jianguang,Sun, Shengming,Ge, Xianping.

[9]Grass carp (Ctenopharyngodon idella) TRAF6 and TAK1: Molecular cloning and expression analysis after Ichthyophthirius multifiliis infection. Zhao, Fei,Li, Yan-Wei,Luo, Xiao-Chun,Li, An-Xing,Zhao, Fei,Pan, Hou-Jun,Wu, Shu-Qin,Shi, Cun-Bin. 2013

[10]Effects of processing method and dietary lysine levels on growth performance, feed conversion ratio and body composition of grass carp, Ctenopharyngodon idella. Gan, Lian,Gan, Lian,Liu, Yong-Jian,Tian, Li-Xia,Liu, Fu-Jia,Yang, Hui-Jun,Chen, Yong-Jun,Liang, Gui-Yin,Yue, Yi-Rong. 2015

[11]Effects of different dietary wheat starch levels on growth, feed efficiency and digestibility in grass carp (Ctenopharyngodon idella). Tian, Li Xia,Liu, Yong Jian,Yang, Hui Jun,Liang, Gui Ying,Niu, Jin. 2012

[12]Dietary magnesium requirements of juvenile grass carp, Ctenopharyngodon idella. Wang, F-B,Luo, L.,Lin, S-M,Li, Y.,Chen, S.,Wang, Y-G,Wen, H.,Hu, C-J. 2011

[13]Tlr22 structure and expression characteristic of barbel chub, Squaliobarbus curriculus provides insights into antiviral immunity against infection with grass carp reovirus. Wang, Rong-hua,Li, Wei,Liu, Qiao-lin,Zeng, Ling-bing,Xiao, Tiao-yi,Fan, Yu-ding,Zeng, Ling-bing.

[14]The influence of feeding rate on growth, feed efficiency and body composition of juvenile grass carp (Ctenopharyngodon idella). Du, Zhen-Yu,Liu, Yong-Jian,Tian, Li-Xia,Ge, Jian-Guo,Cao, Jun-Ming,Liang, Gui-Ying. 2006

[15]Elimination kinetics of eugenol in grass carp in a simulated transportation setting. Zhao, Dong-Hao,Ke, Chang-Liang,Liu, Qi,Wang, Xu-Feng,Wang, Qiang,Li, Liu-Dong,Zhao, Dong-Hao,Ke, Chang-Liang,Liu, Qi,Wang, Xu-Feng,Wang, Qiang,Li, Liu-Dong,Zhao, Dong-Hao,Ke, Chang-Liang,Liu, Qi,Wang, Xu-Feng,Wang, Qiang,Li, Liu-Dong,Zhao, Dong-Hao,Ke, Chang-Liang,Liu, Qi,Wang, Xu-Feng,Wang, Qiang,Li, Liu-Dong. 2017

[16]Effect of Dietary Alanine and Glycine Supplementation on Growth Performance, Body Composition and Apparent Nutrient Digestibility of Juvenile Grass Carp (Ctenopharyngodon idella). Jin, Yan,Li, Sheng-Fa,Cheng, Jia-Hua,Jin, Yan,Li, Sheng-Fa,Cheng, Jia-Hua,Liu, Fu-Jia,Tian, Li-Xia,Liu, Yong-Jian. 2016

[17]Molecular cloning of type I collagen cDNA and nutritional regulation of type I collagen mRNA expression in grass carp. Yu, E. M.,Liu, B. H.,Wang, G. J.,Yu, D. G.,Xie, J.,Xia, Y.,Gong, W. B.,Wang, H. H.,Li, Z. F.,Wei, N..

[18]Genetic parameter estimates for growth of grass carp, Ctenopharyngodon idella, at 10 and 18 months of age. Fu, Jianjun,Shen, Yubang,Xu, Xiaoyan,Li, Jiale,Fu, Jianjun,Li, Jiale.

[19]Effects of dissolved oxygen and dietary lysine levels on growth performance, feed conversion ratio and body composition of grass carp, Ctenopharyngodon idella. Gan, L.,Gan, L.,Liu, Y. -J.,Tian, L. -X.,Yue, Y. -R.,Yang, H. -J.,Liu, F. -J.,Chen, Y. -J.,Liang, G. -Y.,Yue, Y. -R..

[20]Identification of an aminopeptidase from the skeletal muscle of grass carp (Ctenopharyngodon idellus). Liu, Bing-Xin,Sun, Le-Chang,Su, Wen-Jin,Cao, Min-Jie,Zhou, Li-Gen,Hara, Kenji.

作者其他论文 更多>>

微信小程序