Molecular cloning and characterization of lactate dehydrogenase gene from Eimeria tenella

文献类型： 外文期刊

第一作者： Dong, Hui

作者： Dong, Hui;Wang, Yange;Zhao, Qiping;Han, Hongyu;Zhu, Shunhai;Li, Liujia;Wu, Youling;Huang, Bing

作者机构：

关键词： Coccidia;Eimeria;Glycolysis;Invasion;Lactate dehydrogenase

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Lactate dehydrogenase (LDH) is a key enzyme in the glycolytic pathway and is crucial for parasite survival. In this study, we cloned and expressed the LDH of Eimeria tenella (EtLDH). Real-time polymerase chain reaction and Western blot analysis revealed that the expression of EtLDH was developmentally regulated at the messenger RNA (mRNA) and protein levels. EtLDH mRNA levels were higher in second-generation merozoites than in other developmental stages (unsporulated oocysts, sporulated oocysts, and sporozoites). EtLDH protein expression levels were most prominent in second-generation merozoites, moderately expressed in unsporulated oocysts and sporulated oocysts, and weakly detected in sporozoites. Immunostaining with anti-recombinant EtLDH (rEtLDH) antibody indicated that EtLDH was mainly located in the anterior region in free sporozoites and became concentrated in the anterior region of intracellular sporozoites except for the apex after invasion into DF-1 cells. Specific staining of EtLDH protein was more intense in trophozoites and immature first-generation schizonts, but decreased in mature first-generation schizonts. Inhibition of EtLDH function using specific antibodies cannot efficiently reduce the ability of E. tenella sporozoites to invade host cells. These results suggest that EtLDH may be involved in glycolysis during the first-generation merogony stage in E. tenella and has little role in host invasion.

分类号： R53

  • 相关文献

[1]Molecular characterization and protective efficacy of silent information regulator 2A from Eimeria tenella. Dong, Hui,Yang, Sihan,Zhao, Qiping,Han, Hongyu,Zhu, Shunhai,Zhu, Xuelong,Li, Cong,Wang, Ziwen,Xia, Weili,Men, Qifei,Huang, Bing,Yang, Sihan,Yang, Liangyu,Huang, Bing. 2016

[2]Influence of Grape Seed Proanthocyanidin Extract in Broiler Chickens: Effect on Chicken Coccidiosis and Antioxidant Status. Wang, M. L.,Fang, Q.,Wang, X.,Suo, X.,Gu, J. H.,Zhang, W. W.. 2008

[3]Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis. Liu, Lianrui,Huang, Xinmei,Liu, Jianhua,Li, Wenyu,Ji, Yihong,Tian, Di,Tian, Lu,Yang, Xinchao,Xu, Lixin,Yan, Ruofeng,Li, Xiangrui,Song, Xiaokai,Huang, Xinmei. 2017

[4]The complete mitochondrial genomes of five Eimeria species infecting domestic rabbits. Liu, Guo-Hua,Tian, Si-Qin,Zhu, Xing-Quan,Tian, Si-Qin,Wang, Chun-Ren,Zhu, Xing-Quan,Cui, Ping,Fang, Su-Fang.

[5]Isolation of Clostridium perfringens strain W11 and optimization of its biohydrogen production by genetic modification. Wang, Ruofan,Qian, Changli,Wei, Yongjun,Yu, Ruisong,Zhou, Zhihua,Zong, Wenming,Yu, Ruisong.

[6]Effect of hypoxia on the blood of large yellow croaker (Pseudosciaena crocea). Gu Xiaolian,Xu Zhaoli. 2011

[7]Growth and development of testes in domesticated and hybrid (wild X domesticated) yak bulls. Yan, P,Pan, HQ,Zhi, DJ. 2000

[8]Ethanol content in plants of Brassica napus L. correlated with waterlogging tolerance index and regulated by lactate dehydrogenase and citrate synthase. Xu, Benbo,Xu, Benbo,Cheng, Yong,Zou, Xiling,Zhang, Xuekun.

[9]NAD-dependent lactate dehydrogenase catalyses the first step in respiratory utilization of lactate by Lactococcus lactis. Zhao, Rui,Zheng, Sui,Duan, Cuicui,Liu, Fei,Yang, Lijie,Huo, Guicheng,Zhao, Rui,Zheng, Sui. 2013

[10]Effects of protein phosphorylation on color stability of ground meat. Li, Meng,Li, Xin,Xin, Jianzeng,Li, Zheng,Li, Guixia,Zhang, Yan,Du, Manting,Shen, Qingwu W.,Zhang, Dequan,Shen, Qingwu W.. 2017

[11]Phosphoproteomic profiling of myofibrillar and sarcoplasmic proteins of muscle in response to salting. Zhang, Caixia,Wang, Zhenyu,Li, Zheng,Chen, Lijuan,Gao, Lingling,Zhang, Dequan,Shen, Qingwu. 2016

[12]Molecular and biochemical characterization of Eimeria tenella hexokinase. Sun, Mingfei,Liao, Shenquan,Wu, Caiyan,Qi, Nanshan,Lv, Minna,Li, Juan,Lin, Xuhui,Zhang, Jianfei,Xie, Mingquan,Cai, Jianping,Sun, Mingfei,Liao, Shenquan,Wu, Caiyan,Qi, Nanshan,Lv, Minna,Li, Juan,Lin, Xuhui,Zhang, Jianfei,Xie, Mingquan,Cai, Jianping,Sun, Mingfei,Liao, Shenquan,Wu, Caiyan,Qi, Nanshan,Lv, Minna,Li, Juan,Lin, Xuhui,Zhang, Jianfei,Xie, Mingquan,Cai, Jianping,Zhang, Longxian,Zhu, Guan.

[13]Nutritional and physiological responses of finishing pigs exposed to a permanent heat exposure during three weeks. Hao, Yue,Feng, Yuejin,Yang, Peige,Feng, Jinghai,Gu, Xianhong,Lin, Hai.

[14]Proteome analysis of maize seeds: the effect of artificial ageing. Xin, Xia,Lin, Xin-Hai,Chen, Xiao-Ling,Liu, Xu,Lu, Xin-Xiong,Lin, Xin-Hai,Zhou, Yuan-Chang.

[15]Comparative analysis of muscle phosphoproteome induced by salt curing. Wang, Zhenyu,Zhang, Caixia,Li, Zheng,Shen, Qingwu,Zhang, Dequan,Shen, Qingwu,Wang, Zhenyu.

[16]Flexible change and cooperation between mitochondrial electron transport and cytosolic glycolysis as the basis for chilling tolerance in tomato plants. Shi, Kai,Fu, Li-Jun,Zhang, Shuai,Li, Xin,Liao, Yang-Wen-Ke,Xia, Xiao-Jian,Zhou, Yan-Hong,Chen, Zhi-Xiang,Yu, Jing-Quan,Fu, Li-Jun,Wang, Rong-Qing,Yu, Jing-Quan.

[17]Construction and application of EST library from Setaria italica in response to dehydration stress. Zhang, Jinpeng,Liu, Tingsong,Fu, Junjie,Zhu, Yun,Jia, Jinping,Zheng, Jun,Zhao, Yinhe,Zhang, Ying,Wang, Guoying.

[18]Dynamics and regulation of glycolysis-tricarboxylic acid metabolism in the midgut of Spodoptera litura during metamorphosis. Hu, D.,Luo, W.,Fan, L. F.,Liu, F. L.,Gu, J.,Deng, H. M.,Huang, L. H.,Feng, Q. L.,Zhang, C..

[19]High genetic homogeneity points to a single introduction event responsible for invasion of Cotton leaf curl Multan virus and its associated betasatellite into China. Du, Zhenguo,Tang, Yafei,He, Zifu,She, Xiaoman,Du, Zhenguo,Tang, Yafei,He, Zifu,She, Xiaoman. 2015

[20]Tall grasses have an advantage over the invasive vine Mikania micrantha: Potential control agents. Zhou, Sheng,Li, Mingguang,Gao, Lei,Chen, Ping,Wu, Zhi,Xian, Wensheng,Fu, Weidong.

作者其他论文 更多>>

微信小程序