The first case of gynogenesis induced by cold- and heat-shock treatment in Thamnaconus modestus
文献类型: 外文期刊
第一作者: Zhang, Xiaoyan
作者: Zhang, Xiaoyan;San, Lize;Yang, Yucong;Tao, Yuehong;Ren, Jiangong;Liu, Yufeng;He, Zhongwei;Di, Jiashuo;Pei, Ziteng;Wang, Guixing;Hou, Jilun;Zhang, Xiaoyan;San, Lize;Yang, Yucong;Tao, Yuehong;Ren, Jiangong;Liu, Yufeng;He, Zhongwei;Di, Jiashuo;Pei, Ziteng;Wang, Guixing;Hou, Jilun;Di, Jiashuo;Pei, Ziteng
作者机构:
关键词: Thamnaconus modestus; Meiotic gynogenesis; Molecular markers; Homogeneity
期刊名称:AQUACULTURE ( 影响因子:4.5; 五年影响因子:4.6 )
ISSN: 0044-8486
年卷期: 2024 年 580 卷
页码:
收录情况: SCI
摘要: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. The objective of this study was to induce meiotic gynogenesis in Thamnaconus modestus through the application of cold-or heat-shock treatment, with the ultimate goal of establishing a foundational population to enhance breeding outcomes. Firstly, we obtained the optimal dosage of ultraviolet irradiation (55 mJ/cm2) required to render the sperm genome inactive while still allowing egg activation without any contribution of genetic material to the progeny. After fertilization with the inactivated sperm, diploidization was achieved through either cold-shock (12 degrees C for 45 min) or heat-shock (38 degrees C for 1 min), which suppressed the extrusion of the second polar body. Gynogenetic offspring were confirmed through gen-otyping using 12 microsatellite markers with parental specificity. The heterozygosity of meiotic gynogenesis group decreased by 35.14% when analyzed using SNP markers through whole genome resequencing technique, compared with the wild populations. Overall, the meiotic gynogenetic families were successfully induced, and this is the first protocol described for the induction of gynogenesis in T. modestus, which establishing a complete method of meiotic gynogenesis induction. The procedure described herein may be used to produce a clonal line of fish or for the breeding of improved varieties of T. modestus and other related species.
分类号:
- 相关文献
作者其他论文 更多>>
-
Establishment and characterization of a cell line from the brain of the Japanese flounder (Paralichthys olivaceus) and its application in the study of viral infection
作者:Sun, Zhaodi;Yang, Yucong;Fu, Yuanshuai;Sun, Zhaodi;Yang, Yucong;Fu, Yuanshuai;Sun, Zhaodi;Yang, Yucong;Fu, Yuanshuai;Sun, Zhaodi;Ren, Yuqin;Zhang, Yitong;Yang, Yucong;Wang, Guixing;He, Zhongwei;Liu, Yufeng;Cao, Wei;Wang, Yufen;Hou, Jilun
关键词:Paralichthys olivaceus; Cell line; Bohle virus; Chinese giant salamander iridovirus
-
Rapid and specific detection of Chinook salmon bafinivirus (CSBV) in flatfish using loop-mediated isothermal amplification (LAMP)
作者:Su, Huaxing;Su, Huaxing;Zhang, Yitong;He, Zhongwei;Yang, Yucong;Ren, Yuqin;Cao, Wei;Liu, Yufeng;Ren, Jiangong;Wang, Yufen;Wang, Guixing;Hou, Jilun;Su, Huaxing;Zhang, Yitong;He, Zhongwei;Yang, Yucong;Ren, Yuqin;Cao, Wei;Liu, Yufeng;Ren, Jiangong;Wang, Yufen;Wang, Guixing;Hou, Jilun
关键词:Chinook salmon bafinivirus; LAMP; Flatfish disease; Rapid detection method
-
TBBPA and lead co-exposure induces grass carp liver cells apoptosis via ROS/JAK2/STAT3 signaling axis
作者:Li, Lu;Jin, Xin;Yu, Yanbo;Lin, Hongjin;Li, Wan;Liu, Yufeng;Lin, Hongjin
关键词:TBBPA; Pb; Grass carp hepatocytes; ROS/JAK2/STAT3 signaling axis; Apoptosis
-
Population response of intestinal microbiota to acute Vibrio alginolyticus infection in half-smooth tongue sole (Cynoglossus semilaevis)
作者:Hao, Yaotong;Han, Tian;Zhang, Xun;Zhang, Ziying;Gong, Chunguang;Hao, Yaotong;Zhao, Yaxian;Zhang, Yitong;Liu, Yufeng;Wang, Guixing;He, Zhongwei;Cao, Wei;Han, Tian;Wang, Yufen;Gong, Chunguang;Hou, Jilun
关键词:Vibrio alginolyticus; half-smooth tongue sole; secondary infection; intestinal vibrios; metagenomics
-
Genetic diversity and population structure of snap bean (Phaseolus vulgaris L.) from China revealed by microsatellite markers
作者:Hao, Junjie;Song, Fengjing;Cui, Xiao;Wang, Junwei;Zhang, Xiaoyan;Hua, Zeke;Zhu, Tandong;Wu, Zhuobin;Chen, Mingli;Chen, Mingli;Zhang, Xiaoyan
关键词:
-
Integrated Transcriptome and Small RNA Sequencing Analyses Reveals Insights into the Molecular Mechanism of Seed Germination in Mung Bean
作者:Pu, Yanyan;Cong, Yunzhe;Wang, Dong;Gong, Yongchao;Tian, Rumei;Zhang, Xiaoyan;Liu, Min;Ding, Hanfeng;Li, Nana;Wang, Liwen;Xie, Shubin;Li, Leilei;Chen, Xue;Ding, Hanfeng;Li, Nana;Si, Yujun
关键词:Mung bean; transcriptome; differentially expressed genes; miRNA; regulatory network
-
Thyroid Hormone Signaling Is Required for Dynamic Variation in Opsins in the Retina during Metamorphosis of the Japanese Flounder (Paralichthys olivaceus)
作者:Shi, Yaxin;Ji, Wenyao;Li, Xike;Shi, Zhiyi;Li, Wenjuan;Fu, Yuanshuai;Shi, Yaxin;Ji, Wenyao;Li, Xike;Shi, Zhiyi;Li, Wenjuan;Fu, Yuanshuai;Shi, Yaxin;Ji, Wenyao;Li, Xike;Shi, Zhiyi;Li, Wenjuan;Fu, Yuanshuai;Shi, Yang;Hou, Jilun
关键词:Paralichthys olivaceus; opsin; thyroid hormone; metamorphosis