Physicochemical and Fibril Formation Properties of Pufferfish (Takifugu obscurus) Skin Collagen from Solvent Extraction in Different Conditions

文献类型： 外文期刊

第一作者： Wang, Shanshan

作者： Wang, Shanshan;Zhou, Deqing;Liu, Nan;Sun, Yong;Sun, Guohui;Zhou, Deqing;Liu, Nan

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关键词： pufferfish skin; acid-solubilized collagen; pepsin-solubilized collagen; fibril formation; microstructural characteristic

期刊名称：GELS （ 影响因子：4.6； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 9 卷 1 期

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收录情况： SCI

摘要： Acid-solubilized (ASC) and pepsin-solubilized collagen (PSC) extracted at 4 degrees C (ASC-4 and PSC-4), 12 degrees C (ASC-12 and PSC-12), and 20 degrees C (ASC-20 and PSC-20) from the skin of farmed pufferfish (Takifugu obscurus) was characterized by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier-transform infrared spectroscopy (FTIR), and fibril-forming tests. The results indicate that extraction at 12 degrees C can effectively improve the extraction efficiency of natural collagen compared with extraction at 4 degrees C. However, extraction at 20 degrees C results in a decrease in molecular integrity, thus, inducing the resultant collagen to degrade or even lose fibril-forming ability. Transmission electron microscope (TEM) images revealed that ASC-4, PSC-4, ASC-12, and PSC-12 can assemble into fibrils with D-periodicities, and ASC-20 associated into molecular aggregates alongside partial D-banded fibrils, while no well-defined fibrils were observed in PSC-20. Scanning electron microscope (SEM) analysis confirmed the well-defined fibril morphologies of ASC-4, PSC-4, ASC-12, and PSC-12 with imino acid contents between 190.0 and 197.8 residues/1000 residues. The denaturation temperature of ASC-4, PSC-4, ASC-12 and PSC-12 was 30.0, 27.6, 25.9 and 22.7 degrees C, respectively. This study indicates that ASC and PSC extracted at 4 degrees C and 12 degrees C could be alternatives to terrestrial collagens for industrial applications.

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