A novel biosensor-based method for the detection of p-nitrophenol in agricultural soil
文献类型: 外文期刊
第一作者: Ma, Zhao
作者: Ma, Zhao;Ma, Zhao;Lu, Zhongyi;Pan, Jie;Li, Meng;Ma, Zhao;Lu, Zhongyi;Pan, Jie;Li, Meng;Li, Yuanbo
作者机构:
关键词: Biosensors; p -Nitrophenol; pob R1-3 gene; Detection; Soil
期刊名称:CHEMOSPHERE ( 影响因子:8.8; 五年影响因子:8.3 )
ISSN: 0045-6535
年卷期: 2023 年 313 卷
页码:
收录情况: SCI
摘要: Directly measurement of the bioavailable concentration of soil contaminants is essential for their accurate risk assessment. In this study, we successfully modified and identified the key genetic elements (pobR1-3) for the biodetection of p-nitrophenol and synthesized five novel whole-cell biosensors (Escherichia coli BL21/pPNP-mrfp, E. coli BL21/pPNP-CFP, E. coli BL21/pPNP-YFP, E. coli BL21/pPNP-GFP, and E. coli BL21/pPNP-amilCP) to directly detect the concentration of p-nitrophenol in soils. These biosensor methods contained a simple biosensor activation and sample extraction step, a cost-effective detection means, and a fast detection process (5 h) by using a 96-microwell plate with a low background value and high-reliability equation for p-nitrophenol detection. These biosensors had a detection limit of 6.21-25.2 mu g/kg and a linear range of 10-10000 mu g/kg for pnitrophenol in four soils. All biosensors showed better detection performance in the detection of p-nitrophenol in soil samples. The biosensors method can help to quickly and directly assess the actual bioavailable fractions of pnitrophenol in soils, thus facilitating to understand the environmental cycling of p-nitrophenol.
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