A novel viral vaccine platform based on engineered transfer RNA
文献类型: 外文期刊
第一作者: Wang, Tong-Yun
作者: Wang, Tong-Yun;Meng, Fan-Dan;Zhang, Hong-Liang;Tian, Zhi-Jun;Cai, Xue-Hui;Tang, Yan-Dong;Wang, Tong-Yun;Meng, Fan-Dan;Cai, Xue-Hui;Tang, Yan-Dong;Sang, Guo-Ju;Zheng, Hao;Cai, Xue-Hui;Tang, Yan-Dong;Tang, Yan-Dong;Zheng, Hao;Cai, Xue-Hui;Cai, Xue-Hui
作者机构:
关键词: Engineered transfer RNA; PTC virus; vaccine; switch; Pseudorabies virus; novel vaccine
期刊名称:EMERGING MICROBES & INFECTIONS ( 影响因子:13.2; 五年影响因子:9.9 )
ISSN:
年卷期: 2023 年 12 卷 1 期
页码:
收录情况: SCI
摘要: In recent years, an increasing number of emerging and remerging virus outbreaks have occurred and the rapid development of vaccines against these viruses has been crucial. Controlling the replication of premature termination codon (PTC)-containing viruses is a promising approach to generate live but replication-defective viruses that can be used for potent vaccines. Here, we used anticodon-engineered transfer RNAs (ACE-tRNAs) as powerful precision switches to control the replication of PTC-containing viruses. We showed that ACE-tRNAs display higher potency of reading through PTCs than genetic code expansion (GCE) technology. Interestingly, ACE-tRNA has a site preference that may influence its read-through efficacy. We further attempted to use ACE-tRNAs as a novel viral vaccine platform. Using a human immunodeficiency virus type 1 (HIV-1) pseudotyped virus as an RNA virus model, we found that ACE-tRNAs display high potency for read-through viral PTCs and precisely control their production. Pseudorabies virus (PRV), a herpesvirus, was used as a DNA virus model. We found that ACE-tRNAs display high potency for reading through viral PTCs and precisely controlling PTC-containing virus replication. In addition, PTC-engineered PRV completely attenuated and lost virulence in mice in vivo, and immunization with PRV containing a PTC elicited a robust immune response and provided complete protection against wild-type PRV challenge. Overall, replication-controllable PTC-containing viruses based on ACE-tRNAs provide a new strategy to rapidly attenuate virus infection and prime robust immune responses. This technology can be used as a platform for rapidly developing viral vaccines in the future.
分类号:
- 相关文献
作者其他论文 更多>>
-
Corn straw biochar addition elevated phosphorus availability in a coastal salt-affected soil under the conditions of different halophyte litter input and moisture contents
作者:Luo, Xianxiang;Chen, Wenjie;Liu, Qiang;Miao, Jing;Liu, Liuingqing;Zheng, Hao;Liu, Ruhai;Li, Fengmin;Luo, Xianxiang;Zheng, Hao;Liu, Ruhai;Li, Fengmin;Wang, Xiao;Wang, Xiao
关键词:Blue carbon; Soil degradation; Soil health; Litter decomposition; Phosphorous fraction
-
Rotenone impairs brain glial energetics and locomotor behavior in bumblebees
作者:Chen, Jieteng;Liu, Yan;Zheng, Li;Chen, Hao;Zhai, Yifan;Zheng, Hao;Chen, Jieteng;Mu, Xiaohuan;Liu, Huiling;Yong, Qiyao;Ouyang, Xiaoman;Ma, Jie;Meng, Liang;Liu, Shanshan
关键词:Bumblebee; Rotenone; Single -nuclei sequencing; Glial cell; Dopaminergic neuron
-
Hydrochar more effectively mitigated nitrous oxide emissions than pyrochar from a coastal soil of the Yellow River Delta, China
作者:You, Xiangwei;Wang, Xiao;Sun, Ruixue;Fang, Song;Kong, Qingxian;Zhang, Xin;Xie, Chenghao;Li, Yiqiang;Liu, Qiang;Zheng, Hao;Zheng, Hao;Zheng, Hao;Li, Hui
关键词:Salt-affected soils; Soil amendments; Global climate warming; Greenhouse gases; Nitrogen cycle; Denitrification
-
PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein
作者:Zhai, Xueying;Yang, Xia;Zhai, Xueying;Kong, Ning;Wang, Chunmei;Qin, Wenzhen;Dong, Sujie;Zhai, Huanjie;Yang, Xinyu;Ye, Chenqian;Ye, Manqing;Li, Guoxin;Tong, Wu;Liu, Changlong;Zheng, Hao;Yu, Hai;Tong, Guangzhi;Shan, Tongling;Zhang, Wen
关键词:MARCH8; N protein; NDP52; PEDV; PRPF19; selective autophagy
-
Chicken or Porcine Aminopeptidase N Mediates Cellular Entry of Pseudoviruses Carrying Spike Glycoprotein from the Avian Deltacoronaviruses HKU11, HKU13, and HKU17
作者:Liang, Qi-Zhang;Wang, Bin;Ji, Chun-Miao;Feng, Yaoyu;Huang, Yao-Wei;Liang, Qi-Zhang;Hu, Feifan;Liang, Qi-Zhang;Wang, Bin;Ji, Chun-Miao;Qin, Pan;Huang, Yao-Wei;Tang, Yan-Dong
关键词:avian deltacoronavirus; porcine deltacoronavirus (PDCoV); aminopeptidase N (APN); receptor; interspecies transmission
-
PGAM5 degrades PDCoV N protein and activates type I interferon to antagonize viral replication
作者:Yang, Xinyu;Kong, Ning;Qin, Wenzhen;Zhai, Xueying;Song, Yiyi;Tong, Wu;Li, Liwei;Liu, Changlong;Zheng, Hao;Yu, Hai;Tong, Guangzhi;Shan, Tongling;Kong, Ning;Tong, Wu;Liu, Changlong;Zheng, Hao;Yu, Hai;Tong, Guangzhi;Shan, Tongling;Zhang, Wen
关键词:PGAM5; PDCoV; N protein; autophagy; type I interferon
-
N protein of PEDV plays chess game with host proteins by selective autophagy
作者:Zhai, Xueying;Kong, Ning;Song, Yiyi;Qin, Wenzhen;Yang, Xinyu;Ye, Chenqian;Ye, Manqing;Tong, Wu;Liu, Changlong;Zheng, Hao;Yu, Hai;Tong, Guangzhi;Shan, Tongling;Zhai, Xueying;Yang, Xia;Zhang, Gaiping;Zhang, Yu;Zhang, Wen;Tong, Guangzhi;Shan, Tongling
关键词:HNRNPA1; IFN; nucleocapsid protein; PEDV; selective autophagy