Molecular characterization and pathogenicity evaluation of enterovirus G isolated from diarrheic piglets

文献类型： 外文期刊

第一作者： Ibrahim, Yassein M.

作者： Ibrahim, Yassein M.;Wang, Xinrong;Wang, Yue;Ibrahim, Yassein M.;Zhang, Wenli;Werid, Gebremeskel Mamu;Yu, Haidong;Wang, Yue;Fu, Lizhi;Fu, Lizhi;Wang, Yue

作者机构：

关键词： enterovirus G; characterization; genetic recombination; pathogenicity

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Four enterovirus G (EV-G) strains were isolated from diarrheic piglets that were negative for common swine enteric viruses. The spherical enterovirus particles of roughly 30-nm diameter were observed under transmission electron microscopy by using plaque-purified enterovirus. The complete genome sequence analysis revealed that each of four enteroviruses contained a papain-like cysteine protease (PLCP) gene between the 2C and 3A junction regions of the viral genome. This insertion encoded a predicted protease similar to the PLCP of porcine torovirus. The phylogenetic analysis based on complete genome with and without PLCP gene revealed that the four isolated EV-G strains were grouped together with global enterovirus G1-PLCP strains, and more closely related to EV-G/PLCP strains previously detected in China, Japan, and Korea (90.3%-92.2% similarities based on nucleotides). The cell susceptibility test demonstrated that the isolated EV-G could infect and replicate in cell lines from various host species. Furthermore, pathogenicity evaluation showed that the isolated EV-Gs induced mild diarrhea, pyrexia, and reduced body weight in infected piglets. The epidemiological investigation revealed a high prevalence of EV-G in swine herds. Together, our findings demonstrate that the isolated EV-G is pathogenic in piglets and may be advantageous in providing more trustworthy data on the evolution and pathological properties of EV-G.

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