An enzyme-free, ultrasensitive strategy for simultaneous screening of the p-nitrophenyl substituent organophosphorus pesticides
文献类型: 外文期刊
第一作者: Sun, Pengyuan
作者: Sun, Pengyuan;Li, Bingru;Zhao, Jie;Jia, Wenshen;Pan, Ligang;Gong, Wenwen;Liang, Gang;Sun, Pengyuan;Li, Bingru;Zhao, Jie;Jia, Wenshen;Pan, Ligang;Gong, Wenwen;Liang, Gang;Zhen, Jianhui
作者机构:
关键词: Enzyme -free; P-nitrophenyl substituent organophosphorus; pesticides; LC-MS; MS; Indirect detection
期刊名称:FOOD CHEMISTRY ( 影响因子:8.8; 五年影响因子:8.6 )
ISSN: 0308-8146
年卷期: 2023 年 408 卷
页码:
收录情况: SCI
摘要: An enzyme-free, sensitive, and convenient approach was reported for the P-nitrophenyl substituent organophosphorus pesticides (NSOPs) of paraoxon-methyl (PM), paraoxon-ethyl (PE), parathion-methyl (PTM) and parathion-ethyl (PTE)) by indirectly quantification of the 4-nitrophenol (4-NP, hydrolysis product of the NSOPs). NaOH instead of hydrolase/nanozyme was applied, and temperature, pH, ultrasound was investigated to improve the NSOPs hydrolysis. Under the optimized conditions, the hydrolysis efficiencies were up to 99.9 %, 99.9 %, 99.6 %, 96.0 % for PM (10 min), PE (30 min), PTM (90 min) and PTE (120 min), based on which a low detection limits of 0.06 (PM), 0.07 (PE), 0.06 (PTM) and 0.07 (PTE) ppb were calculated with the 4-NP detection limit (0.03 ppb). Furthermore, the method exhibited good performance for the NSOPs with recoveries from 88.87 % to 100.33 % in real samples. This indirect approach offered an ultrasensitive alternative for the NSOPs detection, which holds great potential in practical application for the assessment of food safety and environmental risks.
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