The synergy of thanatin and cathelicidin-BF-15a3 combats Escherichia coli O157:H7
文献类型: 外文期刊
第一作者: Xia, Xuefeng
作者: Xia, Xuefeng;Song, Shiying;Zhang, Shuangyu;Wang, Wei;Zhou, Junming;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Xia, Xuefeng;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Song, Shiying;Dong, Hailong;Li, Bin;Zhang, Xuehan;Luo, Chuping;Li, Bin;Wang, Wei;Zhou, Junming;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Xia, Xuefeng;Song, Shiying;Zhang, Shuangyu;Wang, Wei;Zhou, Junming;Fan, Baochao;Li, Li;Li, Bin;Zhang, Xuehan;Li, Bin;Zhang, Xuehan
作者机构:
关键词: Antimicrobial peptides; Combination use; Food safety; Gram-negative bacteria; Membrane integrity; Vegetables
期刊名称:INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY ( 影响因子:5.4; 五年影响因子:5.5 )
ISSN: 0168-1605
年卷期: 2023 年 386 卷
页码:
收录情况: SCI
摘要: Escherichia coli O157:H7 is a pathogen that commonly causes foodborne illness and represents a health hazard to consumers. The combined use of synergistic antimicrobial peptides (AMPs) is a promising way to improve the microbiological safety of foods. In this study, we detected the synergistic interactions between thanatin and BF -15a3 to reduce their usage and obtain more efficient antibacterial activity. The minimal inhibitory concentra-tions (MICs) of thanatin and BF-15a3 against 49 E. coli O157:H7 strains were ranged from 2 to 8 mu g/mL and 4-32 mu g/mL, showed a general inhibitory effect on E. coli O157:H7 strains, respectively, even multidrug-resistant strains. Their fractional inhibitory concentration index (FICI) was 0.375, which suggested that their combina-tion presented synergistic antibacterial effect against E. coli O157:H7. The killing kinetic curves indicated that the 0.25 x MIC combination had equivalent bactericidal effects to 1 x MIC thanatin or BF-15a3. When AMP combinations were used to treat eukaryotic cells to evaluate the hemolytic characteristics against rabbit eryth-rocytes and cytotoxicity against human embryonic kidney 293T (HEK-293T) cells and intestinal porcine enter-ocyte J2 (IPEC-J2) cells, no magnified adverse effects were observed, exhibiting higher specificity to bacteria and lower toxicity to eukaryotic cells. Compared with bacteriostasis of thanatin or BF-15a3 alone, the proportion of membrane-damaged bacteria treated with the synergetic combination did not appear a significant rise, inter-estingly the Zeta potential of them greatly decreased and their cell membrane permeability significantly increased. Besides, more release of ions and cytoplasm were detected, confirming a more severe loss of mem-brane integrity. These results suggested that the synergistic action mode of thanatin and BF-15a3 is likely attributed to damage aggravation to E. coli membrane. When applying in fresh-cut lettuce and cucumber, their combination allowed for 2.5 log CFU/piece reductions of E. coli O157:H7 in 24 h. In conclusion, the combination of thanatin and BF-15a3 showed excellent synthetic efficacy to kill E. coli O157:H7 in vitro under lower MICs than single use of them.
分类号:
- 相关文献
作者其他论文 更多>>
-
Biocontrol potential of volatile organic compounds produced by Streptomyces corchorusii CG-G2 to strawberry anthracnose caused by Colletotrichum gloeosporioides
作者:Li, Xiaojuan;Zhao, Yankun;Feng, Junting;Chen, Yufeng;Li, Kai;Zhang, Miaoyi;Qi, Dengfeng;Zhou, Dengbo;Wei, Yongzan;Wang, Wei;Xie, Jianghui;Zhang, Lu
关键词:Volatile organic compounds; Strawberry anthracnose; Biological control; Metabolomics; Flavonoids metabolism
-
Responses of lettuce (Lactuca sativa L.) growth and soil properties to conventional non-biodegradable and new biodegradable microplastics
作者:Wang, Weixuan;Xie, Yingmei;Li, Han;Dong, Hongmin;Li, Bin;Guo, Yunjie;Wang, Yutong;Guo, Xinrui;Yin, Tao;Zhou, Weiwei;Liu, Xiaowei
关键词:Microplastics; Poly(butylene adipate; co -terephthalate); Polyethylene; Soil -plant system; Lettuce
-
Comprehensive metabolome and growth effects of thiamethoxam uptake and accumulation from soil on pak choi
作者:Li, Li;Yin, Shijie;Pan, Wei;Wang, Fuyun;Fan, Jiqiao;Li, Li
关键词:Thiamethoxam; Pak choi; Metabolomics; Uptake; Translocation; Plant growth
-
Yield loss of inferior crop species and its physiological mechanism in a semiarid cereal-legume intercropping system
作者:Wang, Wei;Li, Meng-Ying;Zhang, Wei;Rehman, Muhammad Maqsood Ur;Wang, Bao-Zhong;Ullah, Fazal;Cheng, Zheng-Guo;Zhu, Li;Tao, Hong-Yan;Xiong, You-Cai;Zhao, Jian-Hua;Zhang, Jin-Lin;Wang, Wen-Ying;Xiong, You-Cai
关键词:Water competition; Intercropping; Rainfed agroecosystem; Yield formation; Remobilization
-
Immune regulation and inflammation inhibition of Arctium lappa L. polysaccharides by TLR4/NF-κB signaling pathway in cells
作者:Zeng, Feng;Li, Ying;Zhang, Xiaoxiao;Zhao, Xingyu;Huang, Wuyang;Zeng, Feng;Shen, Li;Huang, Wuyang;Zhang, Xiaoxiao;Zhao, Xingyu;Huang, Wuyang;Beta, Trust;Li, Bin;Chen, Rui;Huang, Wuyang
关键词:Arctium lappa L.; Burdock roots; Polysaccharides; Immunoregulation; Inflammation; TLR4/NF-kappa B signaling pathway
-
Fusion of spectra and texture data of hyperspectral imaging for prediction of myoglobin content in nitrite-cured mutton
作者:Wan, Guoling;Liu, Guishan;He, Jianguo;Li, Yan;Guo, Mei;Wan, Guoling;Fan, Shuxiang;Fan, Shuxiang;Wang, Wei;Ma, Chao;Cheng, Lijuan
关键词:Hyperspectral imaging; Texture data; Myoglobin; Spectral dimensionality reduction; Information fusion
-
Folate Biofortification in Soybean: Challenges and Prospects
作者:Agyenim-Boateng, Kwadwo Gyapong;Zhang, Shengrui;Shaibu, Abdulwahab S. S.;Li, Jing;Li, Bin;Sun, Junming;Shohag, Md. Jahidul Islam;Shohag, Md. Jahidul Islam;Shaibu, Abdulwahab S. S.
关键词:soybean (Glycine max L; Merrill); folate; extraction; biofortification; breeding