Comprehensive Evaluation and Transcriptome Analysis Reveal the Salt Tolerance Mechanism in Semi-Wild Cotton (Gossypium purpurascens)
文献类型: 外文期刊
第一作者: Peng, Zhen
作者: Peng, Zhen;Rehman, Abdul;Li, Xiawen;Jiang, Xuran;Tian, Chunyan;Wang, Xiaoyang;Li, Hongge;He, Shoupu;Du, Xiongming;Peng, Zhen;Rehman, Abdul;Li, Hongge;Wang, Zhenzhen;He, Shoupu;Du, Xiongming;Peng, Zhen;He, Shoupu;Du, Xiongming
作者机构:
关键词: Gossypium purpurascens; phenome; receptor-like kinase; salt tolerance evaluation; transcriptome; transcription factor; WGCNA
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.6; 五年影响因子:6.2 )
ISSN: 1661-6596
年卷期: 2023 年 24 卷 16 期
页码:
收录情况: SCI
摘要: Elevated salinity significantly threatens cotton growth, particularly during the germination and seedling stages. The utilization of primitive species of Gossypium hirsutum, specifically Gossypium purpurascens, has the potential to facilitate the restoration of genetic diversity that has been depleted due to selective breeding in modern cultivars. This investigation evaluated 45 G. purpurascens varieties and a salt-tolerant cotton variety based on 34 morphological, physiological, and biochemical indicators and comprehensive salt tolerance index values. This study effectively identified a total of 19 salt-tolerant and two salt-resistant varieties. Furthermore, transcriptome sequencing of a salt-tolerant genotype (Nayanmian-2; NY2) and a salt-sensitive genotype (Sanshagaopao-2; GP2) revealed 2776, 6680, 4660, and 4174 differentially expressed genes (DEGs) under 0.5, 3, 12, and 24 h of salt stress. Gene ontology enrichment analysis indicated that the DEGs exhibited significant enrichment in biological processes like metabolic (GO:0008152) and cellular (GO:0009987) processes. MAPK signaling, plant-pathogen interaction, starch and sucrose metabolism, plant hormone signaling, photosynthesis, and fatty acid metabolism were identified as key KEGG pathways involved in salinity stress. Among the DEGs, including NAC, MYB, WRKY, ERF, bHLH, and bZIP, transcription factors, receptor-like kinases, and carbohydrate-active enzymes were crucial in salinity tolerance. Weighted gene co-expression network analysis (WGCNA) unveiled associations of salt-tolerant genotypes with flavonoid metabolism, carbon metabolism, and MAPK signaling pathways. Identifying nine hub genes (MYB4, MYB105, MYB36, bZIP19, bZIP43, FRS2 SMARCAL1, BBX21, F-box) across various intervals offered insights into the transcriptional regulation mechanism of salt tolerance in G. purpurascens. This study lays the groundwork for understanding the important pathways and gene networks in response to salt stress, thereby providing a foundation for enhancing salt tolerance in upland cotton.
分类号:
- 相关文献
作者其他论文 更多>>
-
Physiological and transcriptional analyses reveal formation of memory under recurring drought stresses in seedlings of cotton (Gossypium hirsutum)
作者:Tian, Zailong;Sun, Yaru;Chen, Baojun;Pan, Zhaoe;Wang, Zhenzhen;Pang, Baoyin;He, Shoupu;Du, Xiongming;Tian, Zailong;Li, Kun;Sun, Yaru;Miao, Yuchen;Tian, Zailong;Du, Xiongming
关键词:Gossypium hirsutum; Drought stress memory; Memory genes; Intergenerational inheritance
-
Physiological and transcriptomic analyses of the effects of coronatine on drought tolerance in Carex leucochlora
作者:Wang, Ye;Shi, Jiannan;Ren, Zhixin;Li, Runzhi;Yu, Chunxin;Peng, Zhen;Gao, Yuerong;Liu, Ziyan;Duan, Liusheng;Fan, Xifeng;Duan, Liusheng
关键词:Drought stress; Plant hormone Coronatine; RNA-seq; Carex
-
Silencing of GhORP_A02 enhances drought tolerance in Gossypium hirsutum
作者:Tajo, Sani Muhammad;Pan, Zhaoe;Jia, Yinhua;He, Shoupu;Chen, Baojun;Sadau, Salisu Bello;Nazir, Mian Faisal;Geng, Xiaoli;Du, Xiongming;Km, Yusuf;Auta, Umar;Ajadi, Aboleri Adijat
关键词:Gossypium hirsutum; Drought; ORP genes; VIGS
-
Multivariate and Association Analyses of Quantitative Attributes Reveal Drought Tolerance Potential of Wheat (Triticum aestivum L.) Genotypes
作者:Khan, Mueen Alam;Akram, Muhammad Waseem;Iqbal, Muhammad;Ahmed, Hafiz Ghulam Muhu-Din;Rehman, Abdul;Iqbal, Hafiz Syed M. Arslan;Alam, Beena
关键词:Drought; correlation; cluster heat map; PCA; tolerance indices; wheat
-
Comparative transcriptome analysis provides insight into the important pathways and key genes related to the pollen abortion in the thermo-sensitive genic male sterile line 373S in Brassica napus L.
作者:Sun, Yanyan;Zhang, Dongsuo;Dong, Hui;Wang, Zhenzhen;Wang, Jing;Lv, Huijie;Guo, Yuan;Hu, Shengwu;Sun, Yanyan;Zhang, Dongsuo;Dong, Hui;Wang, Zhenzhen;Wang, Jing;Lv, Huijie;Guo, Yuan;Hu, Shengwu;Sun, Yanyan
关键词:Brassica napus L; Thermo-sensitive genic male sterility; RNA-sequencing; Cellular events; Differentially expressed genes
-
Genome-wide association study reveals novel genes on different chromosomal positions regulating boll weight in upland cotton (Gossypium hirsutum L.)
作者:Iqbal, Zubair;Iqbal, Muhammad Shahid;Sarfraz, Zareen;Nazir, Mian Faisal;Hu, Daowu;Zafar, Muhammad Mubashar;Mahmood, Tahir;Pan, Zhaoe;Du, Xiongming;Alamery, Salman;Attia, Kotb;Shehzad, Khurram;Fiaz, Sajid;Zaman, Qamar U.;Zaman, Qamar U.;Attacha, Safira;Zeb, Aqib;Gul, Alia M.;Abushady, Asmaa;Abushady, Asmaa;Kimiko, Itoh A.
关键词:Plant bioinformatics; Genome sequence; Candidate genes; Functional analysis; Yield improvement
-
Applications of CRISPR/Cas9 Technology in Ornamental Plants
作者:Liu, Hetong;Liu, Yun;Wei, Yamiao;Zheng, Chengshu;Liu, Hetong;Hu, Zongxia;Wu, Xiang;Wang, Chengpeng;Wang, Zhenzhen;Wang, Zhenzhen;Liu, Hetong;Liu, Yun;Wei, Yamiao;Zheng, Chengshu
关键词:CRISPR/Cas9; Ornamental plants; Applications