Ratiometric Fluorescence Immunoassay Based on Carbon Quantum Dots for Sensitive Detection of Malachite Green in Fish

文献类型： 外文期刊

第一作者： Yang, Guangxin

作者： Yang, Guangxin;Zhang, Jingru;Tang, Yunyu;Zhang, Xuan;Huang, Xuanyun;Shen, Xiaosheng;Kong, Cong;Yang, Guangxin;Zhang, Jingru;Gu, Lin;Zhai, Wenlei;Fodjo, Essy Kouadio

作者机构：

关键词： ratiometric fluorescence; carbon quantum dots; malachite green; immunoassay; 2; 3-diaminophenazine

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN：

年卷期： 2023 年 13 卷 1 期

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收录情况： SCI

摘要： Malachite green (MG) is a synthetic poisonous organic compound that has been banned in many countries as a veterinary drug for aquaculture. An efficient, fast and sensitive method is urgently needed for monitoring the illegal use of malachite green (MG) in aquaculture. In this study, a novel ratiometric fluorescence immunoassay was established. Nitrogen-doped carbon quantum dots were used as ratiometric fluorescent probes with a fluorescence peak at 450 nm. Horseradish peroxidase was employed to convert o-phenylenediamine to 2,3-diaminophenazine, with a new fluorescence peak at 580 nm and a strong absorption at 420 nm. The inner filter effect between N-CQD fluorescence and DAP absorption was identified. It allows for the ratiometric detection of MG using a fluorescent immunoassay. The results demonstrated a linear ratiometric fluorescence response for MG between 0.1 and 12.8 ng center dot mL(-1). The limit of detection of this method was verified to be 0.097 mu g center dot kg(-1) with recoveries ranging from 81.88 to 108%, and the relative standard deviations were below 3%. Furthermore, this method exhibited acceptable consistency with the LC-MS/MS results when applied for MG screening in real samples. These results demonstrated a promising application of this novel ratiometric fluorescence immunoassay for MG screening with the merits of rapid detection, simple sample preparation, and stable signal readout. It can be an alternative to other traditional methods if there are difficulties in the availability of expensive instruments, and achieve comparable results or even more sensitivity than other reported methods.

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