Functional conservation and divergence of SEPALLATA-like genes in floral development in Cymbidium sinense
文献类型: 外文期刊
第一作者: Lin, Zeng-Yu
作者: Lin, Zeng-Yu;Zhu, Gen-Fa;Lu, Chu-Qiao;Gao, Jie;Li, Jie;Xie, Qi;Wei, Yong-Lu;Jin, Jian-Peng;Yang, Feng-Xi;Lin, Zeng-Yu;Wang, Feng-Lan
作者机构:
关键词: C. sinense; SEPALLATA-like genes; expression pattern; ectopic overexpression; transient expression; protein interaction; floral development
期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:5.6; 五年影响因子:6.8 )
ISSN: 1664-462X
年卷期: 2023 年 14 卷
页码:
收录情况: SCI
摘要: Cymbidium sinense is one of the most important traditional Chinese Orchids due to its unique and highly ornamental floral organs. Although the ABCDE model for flower development is well-established in model plant species, the precise roles of these genes in C. sinense are not yet fully understood. In this study, four SEPALLATA-like genes were isolated and identified from C. sinense. CsSEP1 and CsSEP3 were grouped into the AGL9 clade, while CsSEP2 and CsSEP4 were included in the AGL2/3/4 clade. The expression pattern of CsSEP genes showed that they were significantly accumulated in reproductive tissues and expressed during flower bud development but only mildly detected or even undetected in vegetative organs. Subcellular localization revealed that CsSEP1 and CsSEP4 were localized to the nucleus, while CsSEP2 and CsSEP3 were located at the nuclear membrane. Promoter sequence analysis predicted that CsSEP genes contained a number of hormone response elements (HREs) and MADS-box binding sites. The early flowering phenotype observed in transgenic Arabidopsis plants expressing four CsSEP genes, along with the expression profiles of endogenous genes, such as SOC1, LFY, AG, FT, SEP3 and TCPs, in both transgenic Arabidopsis and C. sinense protoplasts, suggested that the CsSEP genes played a regulatory role in the flowering transition by influencing downstream genes related to flowering. However, only transgenic plants overexpressing CsSEP3 and CsSEP4 caused abnormal phenotypes of floral organs, while CsSEP1 and CsSEP2 had no effect on floral organs. Protein-protein interaction assays indicated that CsSEPs formed a protein complex with B-class CsAP3-2 and CsSOC1 proteins, affecting downstream genes to regulate floral organs and flowering time. Our findings highlighted both the functional conservation and divergence of SEPALLATA-like genes in C. sinense floral development. These results provided a valuable foundation for future studies of the molecular network underlying floral development in C. sinense.
分类号:
- 相关文献
作者其他论文 更多>>
-
Adenylate Cyclase Affects the Virulence of Extraintestinal Pathogenic Escherichia coli Derived from Sheep Lungs
作者:Chai, Yingjin;Huang, Xin;Han, Mengli;Zhong, Fagang;Zhang, Xingxing;Wu, Tongzhong;Chai, Yingjin;Gu, Xiaoxiao;Li, Jie;Wang, Xiaolan;Zhou, Xia;Sun, Yingni
关键词:Adhesion and invasion; cyaA; Median lethal dose; XJ10
-
Millisecond self-heating and quenching synthesis of Fe/carbon nanocomposite for superior reductive remediation
作者:Sun, Liming;Wu, Xuan;Jia, Chao;Teng, Tao;Lin, Litao;Yu, Fengbo;He, Zhelin;Gao, Jie;Yan, Shuwen;Zhang, Shicheng;Zhu, Xiangdong;Jiao, Yubing;Yang, Jinguang;Shi, Guosheng;Ren, Zhiyong Jason;Ren, Zhiyong Jason;Zhang, Shicheng;Zhu, Xiangdong
关键词:Fe-0 -based nanomaterials; Flash Joule heating; Reduction; Oxide shell-free; Environmental remediation
-
Application and evaluation of probiotics against red rot disease in Pyropia
作者:Weng, Peiwen;Yang, Huichao;Mo, Zhaolan;Zhang, Wenbin;Yan, Yongwei;Rong, Xiaojun;Li, Jie;Mo, Zhaolan;Yan, Yongwei;Rong, Xiaojun;Li, Jie;Mo, Zhaolan
关键词:Biocontrol; Probiotic; Pseudoalteromonas; Pyropia; Red rot disease
-
Evaluating the Effect on Cultivation of Replacing Soil with Typical Soilless Growing Media: A Microbial Perspective
作者:Li, Jie;Zhou, Wanlai;Yang, Rui;Wang, Hong;Zhang, Dongdong;Li, Yujia;Qi, Zhiyong;Lin, Wei
关键词:bacterial community; fungal community; coir substrate; peat substrate; drip irrigation; tidal irrigation
-
Characteristics of microplastic pollution in golden pompano (Trachinotus ovatus) aquaculture areas and the relationship between colonized- microbiota on microplastics and intestinal microflora
作者:Liu, Ming-Jian;Guo, Hua-Yang;Gao, Jie;Zhu, Ke-Cheng;Guo, Liang;Liu, Bao-Suo;Zhang, Nan;Jiang, Shi-Gui;Zhang, Dian-Chang;Liu, Ming-Jian;Guo, Hua-Yang;Gao, Jie;Zhu, Ke-Cheng;Guo, Liang;Liu, Bao-Suo;Zhang, Nan;Jiang, Shi-Gui;Zhang, Dian-Chang;Liu, Ming-Jian;Guo, Hua-Yang;Gao, Jie;Zhu, Ke-Cheng;Guo, Liang;Liu, Bao-Suo;Zhang, Nan;Jiang, Shi-Gui;Zhang, Dian-Chang;Jiang, Shi-Gui;Zhang, Dian-Chang;Jiang, Shi-Gui;Zhang, Dian-Chang;Liu, Ming-Jian
关键词:Microbial community; Beibu Gulf; Microplastics (MPs); Trachinotus ovatus; Gut microbiome
-
Isoprenylation modification is required for HIPP1-mediated powdery mildew resistance in wheat
作者:Wang, Zongkuan;Zhang, Heng;Li, Yingbo;Chen, Yiming;Tang, Xiong;Zhao, Jia;Yu, Feifei;Wang, Haiyan;Xiao, Jin;Liu, Jia;Zhang, Xu;Sun, Li;Xie, Qi;Wang, Xiue;Wang, Zongkuan;Zhang, Heng;Li, Yingbo;Zhang, Heng;Li, Yingbo
关键词:heavy-metal-associated protein; plant defence signalling; protein isoprenylation modification; Triticum aestivum L
-
Identification of insect sources of honey in China based on real-time fluorescent LAMP technology
作者:Gao, Jie;Jin, Xiue;Gong, Bo;Li, Jingjing;Wang, Jun;Gao, Jie;Li, Jingjing;Gao, Jie;Chen, Ailiang;Tan, Jianxin;Wang, Jun
关键词:Apis mellifera; Apis cerana; Honey; Loop -mediated isothermal amplification; (LAMP); Major Royal Jelly Protein 2 ( MRJP2 )