PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer
文献类型: 外文期刊
第一作者: Wang, Jialing
作者: Wang, Jialing;Chen, Yan;He, Xiaohua;Du, Xiaoli;Gao, Yongheng;Shan, Xinggen;Hu, Zhiqun;Hu, Qinghai
作者机构:
关键词: Riemerella anatipestifer; protective antigen; subcellular localization; the type IX secretion system (T9SS); PaR1
期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:5.2; 五年影响因子:6.2 )
ISSN:
年卷期: 2023 年 13 卷
页码:
收录情况: SCI
摘要: Riemerella anatipestifer mainly infects domestic ducks, geese, turkeys, and other birds, and causes considerable economic losses to the global duck industry. Previous studies have shown that concentrated cell-free culture filtrates of R. anatipestifer induce highly significant protection against homologous challenge. In this study, 12 immunogenic proteins were identified in the culture supernatant of R. anatipestifer strain Yb2 with immunoproteomic analysis. Of these, three immunogenic proteins, AS87_RS06600 (designated "PaR1" in this study), AS87_RS09020, and AS87_RS09965, which appeared in more than three spots on the western-blotted membrane, were expressed in Escherichia coli and purified. Animal experiments showed that the recombinant PaR1 (rPaR1) protein protected 41.67% of immunized ducklings against challenge with virulent Yb2, whereas rAS87_RS09020 or rAS87_RS09965 did not, and that ducklings immunized once with rPaR1 were 20, 40, and 0% protected from challenge with R. anatipestifer strains WJ4 (serotype 1), Yb2 (serotype 2), and HXb2 (serotype 10), respectively. In addition, rPaR1 immunized rabbit serum showed bactericidal activity against strain Yb2 at a titer of 1:8. These results indicate that rPaR1 of strain Yb2 protects against homologous challenge. Amino acid homology analysis show that PaR1 is a non-serotype-specific protein among different R. anatipestifer serotypes. Furthermore, PaR1 is mainly secreted outside the cell through the T9SS. Overall, our results demonstrate that R. anatipestifer PaR1 is a non-serotype-specific protective protein secreted by the T9SS.
分类号:
- 相关文献
作者其他论文 更多>>
-
DEAD Box Protein DhR1 Is a Global Regulator Involved in the Bacterial Fitness and Virulence of Riemerella anatipestifer
作者:Du, Xiaoli;Wang, Jialing;Shan, Xinggen;Hu, Zhiqun;Gao, Yongheng;Hu, Qinghai
关键词:Riemerella anatipestifer; DEAD box protein; bacterial fitness; virulence
-
Hybrid bismuth oxide-graphine oxide nanomaterials improve the signal-to-noise response of small molecules analyzed by matrix assisted laser desorption ionization-time-of-flight mass spectrometry
作者:Tang, Xuemei;Chen, Zhiting;Chen, Yan;Jiang, Xunyuan;Zhu, Fuwei;Liu, Shuai;Wan, Kai;Tang, Xuemei;Zhu, Fuwei;Wan, Kai;Chen, Yan;Liu, Shuai;Wan, Kai
关键词:MALDI; Small molecules; Nanomaterial; Time-of-flight; Mass spectrometry
-
Comparative analysis of carcass traits and meat quality in indigenous Chinese cattle breeds
作者:Ge, Fei;Li, Junya;Gao, Han;Wang, Xiujuan;Zhang, Xiaobei;Gao, Huijiang;Zhang, Lupei;Xu, Lingyang;Gao, Xue;Zhu, Bo;Wang, Zezhao;Chen, Yan;Zhang, Xiaobei;Chen, Yan
关键词:Carcass characteristic; Meat quality; Nutritional ingredients; Amino acids composition; Fatty acids composition; Indigenous Chinese cattle breeds; Beef industry
-
Orientation-driven photosynthesized carbon belowground mediates intercropped peanut microbiota changes for pathogen resistance
作者:Lu, Jumeng;He, Ganghui;Li, Shiwen;Sun, Bo;Chen, Yan;Lu, Jumeng;Shen, Yi;He, Ganghui;Li, Shiwen;Kumar, Amit;Kumar, Amit
关键词:Photosynthesized carbon assimilation; Photosynthetically active radiation; Rhizosphere microbial functioning; Plant growth promotion; Pathogen defence; Burkholderia
-
The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC
作者:Chen, Rongrong;Chen, Rongrong;Liu, Yan;Chen, Yan;Liu, Yunjun;Wei, Qianhan;Du, Xuemei;Wang, Jianhua;Li, Jiankun
关键词:Maize; Empty pericarp 601; PPR; Mitochondrial; Ccmc; Seed development
-
Progesterone and Androstenedione Are Important Follicular Fluid Factors Regulating Porcine Oocyte Maturation Quality
作者:Zhao, Huaxing;He, Xiaohua;Zhang, Xianjun;Cai, Gengyuan;Huang, Sixiu;Xu, Zheng;Wu, Zhenfang;Li, Zicong;Zhao, Huaxing;He, Xiaohua;Zhang, Xianjun;Cai, Gengyuan;Huang, Sixiu;Xu, Zheng;Wu, Zhenfang;Li, Zicong;Zhao, Huaxing;He, Xiaohua;Zhang, Xianjun;Cai, Gengyuan;Huang, Sixiu;Xu, Zheng;Wu, Zhenfang;Li, Zicong;Zhao, Huaxing;He, Xiaohua;Zhang, Xianjun;Shi, Junsong;Cai, Gengyuan;Huang, Sixiu;Xu, Zheng;Wu, Zhenfang;Li, Zicong;Shi, Junsong;Zhou, Rong;Mai, Ranbiao;Su, Qiaoyun;Wu, Zhenfang;Li, Zicong
关键词:pig; oocyte maturation; follicular fluid; maturation medium; metabolomics
-
Changes in the characteristic volatile aromatic compounds in tuna cooking liquid during fermentation and deodorization by Lactobacillus plantarum RP26 and Cyberlindnera fabianii JGM9-1
作者:Ma, Wenjing;Liang, Zhangcheng;He, Bing;He, Zhigang;Chen, Bingyan;Lin, Xiaozi;Ma, Wenjing;Liang, Zhangcheng;He, Bing;Wu, Yuxi;He, Zhigang;Chen, Bingyan;Lin, Xiaozi;Liang, Zhangcheng;He, Zhigang;Chen, Bingyan;Lin, Xiaozi;Chen, Yan;Luo, Lianyu;Chen, Yan;Luo, Lianyu;He, Zhigang;Lin, Xiaozi
关键词:Tuna cooking liquid; Characteristic volatile aromatic compounds; Microbial deodorization; Cyberlindnera fabianii JGM9-1; Lactobacillus plantarum RP26