Molecular basis of the plant ROS1-mediated active DNA demethylation

文献类型： 外文期刊

第一作者： Du, Xuan

作者： Du, Xuan;Xie, Guohui;Wang, Changshi;Yan, Kaige;Zhu, Jian-Kang;Du, Jiamu;Du, Xuan;Li, Sisi;Yang, Zhenlin;Yang, Zhenlin;Zhang, Laixing;Yang, Maojun;Zhu, Jian-Kang;Zhu, Jian-Kang

作者机构：

期刊名称：NATURE PLANTS （ 影响因子：18.0； 五年影响因子：18.6 ）

ISSN： 2055-026X

年卷期： 2023 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Active DNA demethylation plays a crucial role in eukaryotic gene imprinting and antagonizing DNA methylation. The plant-specific REPRESSOR OF SILENCING 1/DEMETER (ROS1/DME) family of enzymes directly excise 5-methyl-cytosine (5mC), representing an efficient DNA demethylation pathway distinct from that of animals. Here, we report the cryo-electron microscopy structures of anArabidopsisROS1 catalytic fragment in complex with substrate DNA, mismatch DNA and reaction intermediate, respectively. The substrate 5mC is flipped-out from the DNA duplex and subsequently recognized by the ROS1 base-binding pocket through hydrophobic and hydrogen-bonding interactions towards the 5-methyl group and Watson- Crick edge respectively, while the different protonation states of the bases determine the substrate preference for 5mC over T:G mismatch. Together with the structure of the reaction intermediate complex, our structural and biochemical studies revealed the molecular basis for substrate specificity, as well as the reaction mechanism underlying 5mC demethylation by the ROS1/ DME family of plant-specific DNA demethylases.

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