Molecular mapping of YrTZ2, a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with Aegilops tauschii

文献类型： 外文期刊

第一作者： Wang Zhen-zhong

作者： Wang Zhen-zhong;Xie Jing-zhong;Chen Yong-xing;Wu Qiu-hong;Lu Ping;Wu Hai-bin;Zhang Huai-zhi;Liu Zhi-yong;Guo Li;Zhang De-yun;Li Miao-miao;Zhang Yan;Li Gen-qiao;Fang Ti-lin;Li Jun;Yang Wu-yun;Wu Hai-bin;Luo Ming-cheng;Tzion, Fahima

作者机构：

关键词： Triticum dicoccoides; Puccinia striiformis f. sp tritici; SNP; comparative genomics

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Wheat stripe rust, caused by Puccinia striiiformis f. sp. tritici (Pst), is a devastating disease that can cause severe yield losses. Identification and utilization of stripe rust resistance genes are essential for effective breeding against the disease. Wild emmer accession TZ-2, originally collected from Mount Hermon, Israel, confers near-immunity resistance against several prevailing Pst races in China. A set of 200 F-6.7 recombinant inbred lines (RILs) derived from a cross between susceptible durum wheat cultivar Langdon and TZ-2 was used for stripe rust evaluation. Genetic analysis indicated that the stripe rust resistance of TZ-2 to Pst race CYR34 was controlled by a single dominant gene, temporarily designated YrTZ2. Through bulked segregant analysis (BSA) with SSR markers, YrTZ2 was located on chromosome arm 1BS flanked by Xwmc230 and Xgwm413 with genetic distance of 0.8 cM (distal) and 0.3 cM (proximal), respectively. By applying wheat 90K iSelect SNP genotyping assay, 11 polymorphic loci (consisting of 250 SNP markers) closely linked to YrTZ2 were identified. YrTZ2 was further delimited into a 0.8-cM genetic interval between SNP marker 1WB19368 and SSR marker Xgwm413, and co-segregated with SNP marker IWB28744 (co-segregated with 28 SNP). Comparative genomics analyses revealed high level of collinearity between the Yr TZ2 genomic region and the orthologous region of Aegilops tauschii 1DS. The genomic region between loci IW1319368 and IWB31649 harboring YrTZ2 is orthologous to a 24.5-Mb genomic region between AT1D0112 and AT1D0150, spanning 15 contigs on chromosome 1DS. The genetic and comparative maps of YrTZ2 provide a framework for map-based cloning and marker-assisted selection of YrTZ2.

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