Smartphone-based chemiluminescence detection of aflatoxin B1 via labelled and label-free dual sensing systems

文献类型： 外文期刊

第一作者： Ma, Junning

作者： Ma, Junning;Xing, Fuguo;Li, Xu;Guan, Yue;Wang, Yan;Yu, Qiang;Yu, Xiaohua

作者机构：

关键词： Smartphone; Chemiluminescence; Label-free; Split aptamer; Split DNAzyme

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 413 卷

页码：

收录情况： SCI

摘要： To develop a sensing platform for onsite determination of AFB1 in foodstuffs, we developed smartphone-based chemiluminescence detection of AFB1 via labelled and label-free dual modes. The labelled mode was charac-teristic of double streptavidin-biotin mediated signal amplification, obtaining limit of detection (LOD) of 0.04 ng/mL in the linear range of 1-100 ng/mL. To reduce the complexity in the labelled system, a label-free mode based on both split aptamer and split DNAzyme was fabricated. A satisfactory LOD of 0.33 ng/mL was generated in the linear range of 1-100 ng/mL. Both labelled and label-free sensing systems achieved outstanding recovery rate in AFB1-spiked maize and peanut kernel samples. Finally, two systems were successfully integrated into smartphone-based portable device based on custom-made components and android application, achieving comparable AFB1 detection ability to a commercial microplate reader. Our systems hold huge potential for AFB1 onsite detection in food supply chain.

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