DNA methylation reprogramming provides insights into light-induced anthocyanin biosynthesis in red pear

文献类型： 外文期刊

第一作者： Liu, Hai-Nan

作者： Liu, Hai-Nan;Pei, Mao-Song;Wu, Jun;Liu, Hai-Nan;Pei, Mao-Song;Shu, Qun;Su, Jun;Kui, Lin-Wang;Espley, Richard V.;Allan, Andrew C.;Allan, Andrew C.;Li, Xiao-Long;Wu, Jun

作者机构：

关键词： Light; Red-skinned pear; Anthocyanin; Differentially Methylated Regions; Differentially Expressed Genes; BS-PCR

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2023 年 326 卷

页码：

收录情况： SCI

摘要： DNA methylation, an epigenetic mark, is proposed to regulate plant anthocyanin biosynthesis. It well known that light induces anthocyanin accumulation, with bagging treatments commonly used to investigate light-controlled anthocyanin biosynthesis. We studied the DNA methylome landscape during pear skin coloration under various conditions (fruits re-exposed to sunlight after bag removal). The DNA methylation level in gene body/TE and its flanking sequence was generally similar between debagged and bagged treatments, however differentially methylated regions (DMRs) were re-modelled after light-exposure. Both DNA demethylase homologs and the RNA-directed DNA methylation (RdDM) pathways contributed to this re-distribution. A total of 310 DEGs were DMR-associated during light-induced anthocyanin biosynthesis between debagged and bagged treatments. The hypomethylated mCHH context was seen within the promoter of PyUFGT, together with other anthocyanin biosynthesis genes (PyPAL, PyDFR and PyANS). This enhanced transcriptional activation and promoted antho-cyanin accumulation after light re-exposure. Unlike previous reports on bud sports, we did not detect DMRs within the MYB10 promoter. Instead, we observed the genome-wide re-distribution of methylation patterns, suggesting different mechanisms underlying methylation patterns of differentially accumulated anthocyanins caused by either bud mutation or environment change. We investigate the dynamic landscape of genome-scale DNA methylation, which is the combined effect of DNA demethylation and RdDM pathway, in the process of light-induced fruit colour formation in pear. This process is regulated by methylation changes on promoter re-gions of several DEGs. These results provide a DMR-associated DEGs set and new insight into the mechanism of DNA methylation involved in light-induced anthocyanin biosynthesis.

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