Inhibitory effects of lactoferrin on pulmonary inflammatory processes induced by lipopolysaccharide by modulating the TLR4-related pathway
文献类型: 外文期刊
第一作者: Li, H. Y.
作者: Li, H. Y.;Wu, H. M.;Yao, Q. Q.;Zhang, Z. Y.;Fan, L. L.;Wang, J. Q.;Zheng, N.;Li, H. Y.;Wu, H. M.;Yao, Q. Q.;Zhang, Z. Y.;Meng, Q. S.;Fan, L. L.;Wang, J. Q.;Zheng, N.;Yang, H. G.
作者机构:
关键词: lung inflammation; TLR-4; lactoferrin
期刊名称:JOURNAL OF DAIRY SCIENCE ( 影响因子:4.034; 五年影响因子:4.354 )
ISSN: 0022-0302
年卷期: 2021 年 104 卷 7 期
页码:
收录情况: SCI
摘要: This study tested the ability of lactoferrin to modu-late pulmonary inflammation. To construct in vitro and in vivo inflammatory lung models, cells from the human lung adenocarcinoma cell line (A549) were exposed to lipopolysaccharide (LPS, 1 & micro;g/mL), and mice (CD-1) were intratracheally administered LPS [10 mg/kg of body weight (BW), tracheal lumen injec-tion], respectively. The A549 cells were preincubated with lactoferrin (10 mg/mL), and the mice were in-traperitoneally injected with lactoferrin (100 mg/kg of BW), followed by LPS treatment. The concentrations of proinflammatory cytokines (IL-1 beta and TNF-alpha) in culture medium of A549 cells and in bronchoalveolar lavage fluid of the mice were determined using enzyme-linked immunosorbent assays. The toll-like receptor 4-related pathway (TLR4/MyD88/IRAK1/TRAF6/ NF kappa B) was determined at gene and protein expression levels in A549 cells and mouse lung tissue. Results showed that LPS treatment significantly elevated the concentrations of IL-1 beta and TNF-alpha in the A549 cell culture medium and in bronchoalveolar lavage fluid of the mice; it also elevated both the mRNA and protein expressions of TLR4 and the TLR4 downstream factors in A549 cells and mouse lung tissue. Nevertheless, lac-toferrin apparently depressed the releases of IL-1 beta and TNF-alpha from A549 cells and lung tissues stimulated by LPS, and significantly suppressed the TLR4 signaling pathway. Lactoferrin also promoted the enhancement of miR-146a expression in A549 cells and mouse lung tissue. Moreover, 100 degrees C heating for 3 min caused total loss of the previously listed bioactivity of lactoferrin. Collectively, we proved that lactoferrin intervened inLPS-induced inflammation in the pulmonary cell model and in the mouse model, through inhibiting the TLR4- related pathway.
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