RNA sequencing reveals CircRNA expression profiles in chicken embryo fibroblasts infected with velogenic Newcastle disease virus
文献类型: 外文期刊
第一作者: Chen, Libin
作者: Chen, Libin;Ruan, Jiayu;Chen, Yiyi;Deng, Wenxuan;Lai, Jinyu;Fan, Lei;Cai, Juncheng;Lin, Qiuyan;Ren, Tao;Chen, Libin;Ruan, Jiayu;Chen, Yiyi;Deng, Wenxuan;Lai, Jinyu;Fan, Lei;Cai, Juncheng;Lin, Qiuyan;Ren, Tao;Chen, Libin;Ruan, Jiayu;Chen, Yiyi;Deng, Wenxuan;Lai, Jinyu;Fan, Lei;Cai, Juncheng;Lin, Qiuyan;Ren, Tao;Chen, Libin;Ruan, Jiayu;Chen, Yiyi;Deng, Wenxuan;Lai, Jinyu;Fan, Lei;Cai, Juncheng;Lin, Qiuyan;Ren, Tao;Ding, Chan;Xiang, Bin
作者机构:
关键词: chicken embryo fibroblasts; Newcastle disease virus; RNA sequencing; infection; circular RNA
期刊名称:FRONTIERS IN VETERINARY SCIENCE ( 影响因子:3.2; 五年影响因子:3.5 )
ISSN:
年卷期: 2023 年 10 卷
页码:
收录情况: SCI
摘要: IntroductionNewcastle disease virus (NDV) is an important avian pathogen prevalent worldwide; it has an extensive host range and seriously harms the poultry industry. Velogenic NDV strains exhibit high pathogenicity and mortality in chickens. Circular RNAs (circRNAs) are among the most abundant and conserved eukaryotic transcripts. They are part of the innate immunity and antiviral response. However, the relationship between circRNAs and NDV infection is unclear. MethodsIn this study, we used circRNA transcriptome sequencing to analyze the differences in circRNA expression profiles post velogenic NDV infection in chicken embryo fibroblasts (CEFs). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to reveal significant enrichment of differentially expressed (DE) circRNAs. The circRNA- miRNA-mRNA interaction networks were further predicted. Moreover, circ-EZH2 was selected to determine its effect on NDV infection in CEFs. ResultsNDV infection altered circRNA expression profiles in CEFs, and 86 significantly DE circRNAs were identified. GO and KEGG enrichment analyses revealed significant enrichment of DE circRNAs for metabolism-related pathways, such as lysine degradation, glutaminergic synapse, and alanine, aspartic-acid, and glutamic-acid metabolism. The circRNA- miRNA-mRNA interaction networks further demonstrated that CEFs might combat NDV infection by regulating metabolism through circRNA-targeted mRNAs and miRNAs. Furthermore, we verified that circ-EZH2 overexpression and knockdown inhibited and promoted NDV replication, respectively, indicating that circRNAs are involved in NDV replication. ConclusionsThese results demonstrate that CEFs exert antiviral responses by forming circRNAs, offering new insights into the mechanisms underlying NDV-host interactions.
分类号:
- 相关文献
作者其他论文 更多>>
-
Potassium deficiency stress reduces Rubisco activity in Brassica napus leaves by subcellular acidification decreasing photosynthetic rate
作者:Hu, Wenshi;Gu, Hehe;Wang, Kunjiao;Lu, Zhifeng;Li, Xiaokun;Cong, Rihuan;Ren, Tao;Lu, Jianwei;Hu, Wenshi;Ren, Tao
关键词:Potassium; Rubisco; Photosynthesis; pH regulation; Brassica napus; Subcellular
-
NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway
作者:Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Xiang, Bin;Ding, Chan
关键词:Autophagy; inflammation; mitochondrial damage; mitophagy; Newcastle disease virus
-
Single-Cell Transcriptome Atlas of Newcastle Disease Virus in Chickens Both In Vitro and In Vivo
作者:Liu, Weiwei;Qiu, Yafeng;Qiu, Xusheng;Tan, Lei;Song, Cuiping;Sun, Yingjie;Liao, Ying;Ding, Chan;Xu, Zejun;Liu, Xiufan;Ding, Chan
关键词:Newcastle disease virus; single cell; chicken; interferon response; transcript
-
Cellular vimentin regulates the infectivity of Newcastle disease virus through targeting of the HN protein
作者:Lu, Xiaolong;Liu, Kaituo;Chen, Yu;Gao, Ruyi;Hu, Zenglei;Hu, Jiao;Gu, Min;Hu, Shunlin;Jiao, Xinan;Wang, Xiaoquan;Liu, Xiufan;Liu, Xiaowen;Hu, Zenglei;Hu, Jiao;Gu, Min;Hu, Shunlin;Jiao, Xinan;Wang, Xiaoquan;Liu, Xiufan;Liu, Xiaowen;Ding, Chan;Jiao, Xinan;Liu, Xiufan;Hu, Zenglei;Jiao, Xinan;Ding, Chan;Jiao, Xinan
关键词:Newcastle disease virus; HN; vimentin; viral infection; macrophage
-
A synthesis-analysis of winter oilseed rape yield response to planting density under intensive cropping system in Yangtze River Basin
作者:Cong, Rihuan;Zhong, Zijing;Zhang, Zhi;Liu, Yu;Lu, Zhifeng;Ren, Tao;Li, Xiaokun;Lu, Jianwei;Zhang, Zhi;Lu, Jianwei
关键词:
-
Newcastle Disease Virus Manipulates Mitochondrial MTHFD2-Mediated Nucleotide Metabolism for Virus Replication
作者:Tang, Ning;Zhao, Changrun;Luo, Tingrong;Ding, Chan;Tang, Ning;Tan, Lei;Song, Cuiping;Qiu, Xusheng;Liao, Ying;Sun, Yingjie;Ding, Chan;Chen, Pingyi;Liu, Panrao;Liu, Xiufan;Ding, Chan;Luo, Tingrong
关键词:MTHFD2; Newcastle disease virus; c-Myc; mitochondrial 1C metabolism; oxPPP
-
Chicken-derived MERTK protein inhibits Newcastle disease virus replication by increasing STAT1 phosphorylation in DF-1 cells
作者:Tan, Lei;Huang, Meizhen;Qiu, Xusheng;Zhi, Xuechun;Liang, Lujing;Sun, Yingjie;Liao, Ying;Song, Cuiping;Ding, Chan;Tan, Lei;Ren, Tao;Ding, Chan
关键词:Receptor tyrosine kinase; MERTK; Newcastle disease virus (NDV); Signal transducer and activator of transcription; 1 (STAT1); Casitas B -lineage proto-oncogene (CBL)