Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton

文献类型： 外文期刊

第一作者： Jiang, Ming-feng

作者： Jiang, Ming-feng;Li, Sheng-wei;Chen, Min;Li, Sheng-wei;Chen, Min;Cai, Ying-fan;Xie, Yong-fang;Li, Biao;Sun, Quan;Jiang, Huai-zhong;Pan, Zheng;Gao, Yun-ling;Yuan, You-Lu;Shi, Yu-zheng

作者机构：

关键词： Methods and Techniques;Molecular Genetics: Biochemistry and Molecular Biophysics;Agronomy: Agriculture

期刊名称：JOURNAL OF PLANT BIOLOGY （ 影响因子：2.434； 五年影响因子：2.455 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A cDNA encoding a novel cysteine proteinase inhibitor (CPI) was isolated from a gland mutant Xiangmian-18 of upland cotton during the pigments gland forming stage. The cDNA comprises 378 bp and encodes 125 amino acid residues with molecular mass of 13.8 kDa. It contains the conserved motif of cysteine protease inhibitors and belongs to the cystatin superfamily (Gln-Val-Val-Ala-Gly). The deduced amino acid sequences of the domains are highly similar to the normal upland cotton (96.8%). SDS-PAGE and western hybridization analysis showed that the expressed recombinant protein was recombinant CPI. The inhibitory activity of recombinant CPI was 46 u/mu g which was measured by inhibiting the protease activity of papain. RT-PCR results indicated that the expression level of developing gland stage was higher than that of undeveloped gland stage.

分类号： Q94

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