Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

文献类型： 外文期刊

第一作者： Hao, Jia

作者： Hao, Jia;Li, Yihang;Wang, Jingxuan;Liu, Xianjin;Hao, Jia;Li, Yihang;Wang, Jingxuan;Xu, Chongxin;Gao, Meijing;Chen, Wei;Zhang, Xiao;Hu, Xiaodan;Liu, Yuan;Liu, Xianjin;Hao, Jia;Li, Yihang;Wang, Jingxuan;Xu, Chongxin;Gao, Meijing;Chen, Wei;Zhang, Xiao;Hu, Xiaodan;Liu, Yuan;Liu, Xianjin

作者机构：

关键词： Bacillus thuringiensis; Cry1F toxin; anti-idiotype nanobody; Ostrinia furnacalis (Guenee); brush border membrane vesicles (BBMV)

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2020 年 31 卷 1 期

页码：

收录情况： SCI

摘要： Anti-idiotypic antibody technology has been widely developed. Here, we prepared the F(ab')(2) fragments of the Cry1F toxin polyclonal antibody as Ab1and screened an anti-idiotype nanobody (VHH) for Bt Cry1F toxin from the camelid naive antibody phage display library. After three cycles of panning, six clones were isolated and subjected to indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for the detection of the Cry1F toxin. A competition inhibition rate of 47.00% exists between the anti-idiotype nanobody with the highest activity (5B) and Cry1F toxin for F(ab')(2) fragments. The binding abilities between the anti-idiotypic nanobody 5B and brush border membrane vesicles (BBMV) of Ostrinia furnacalis (Guenee) are also good. The anti-idiotypic nanobody 5B expressed through the prokaryotic system has the same activity as in phage form, and mortality compared to controls could reach 23.3% (P < 0.05). The material has the potential to simulate the Cry1F toxin to a certain extent.

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