The effects of temperature changes on the isozyme and Hsp70 levels of the Amur sturgeon, Acipenser schrenckii, at two acclimation temperatures
文献类型: 外文期刊
第一作者: Luo, Liang
作者: Luo, Liang;Zhao, Zhigang;Zhang, Rui;Guo, Kun;Wang, Shihui;Xu, Wei;Wang, Changan
作者机构:
关键词: Amur sturgeon, Acipenser schrenckii; Hsp70; Isozymes; Temperature changes
期刊名称:AQUACULTURE ( 影响因子:5.135; 五年影响因子:5.125 )
ISSN: 0044-8486
年卷期: 2022 年 551 卷
页码:
收录情况: SCI
摘要: The isozyme spectrum expressions and Hsp70 expressions in the muscle and the isozyme activities in the liver were measured to evaluate the stress response of the Amur sturgeon, Acipenser schrenckii , to rapid temperature changes at two acclimation temperatures. The fish (body weight 6.18 & PLUSMN; 0.21 g) were subjected to an increasing temperature change (from 13 ? to 23 ?, Tinc treatment, 60 individuals) or to a decreasing temperature change (from 23 ? to 13 ?, Tdec treatment, 60 individuals) for 72 h. After 0 h, 1 h, 3 h, 12 h, 24 h, 48 h and 72 h of exposure, the fish (n = 7) were removed and prepared for further analysis. The results showed that the levels of Hsp70 in the muscle were significantly upregulated after 48 h of heat exposure (P < 0.05) in the Tinc treatment; however, the level of Hsp70 increased quickly after 1 h of cold exposure (P < 0.05) and returned to initial levels after 3 h of cold exposure in the Tdec treatment (P < 0.05). The expressed levels of alkaline phosphatase (ALP), superoxide dismutase (SOD), lactate dehydrogenase (LDH), acid phosphatase (ACP) and esterase (EST) in the muscle showed significant changes in both the Tinc and Tdec treatments (P < 0.05). The activities of ALP, LDH, ACP and peroxidase (POD) in the liver were significantly lower in the Tinc treatment than that in the Tdec treatment at 0 h after exposure (P < 0.05). The activities of ALP, SOD, LDH, ACP and POD in the liver showed significant changes after different heat or cold exposure times (P < 0.05). The results suggest that qualitative and quantitative changes in isozymes and Hsp70 during temperature changes may significantly contribute to the metabolic reorganization that occurs during temperature acclimation, which imply that the Amur sturgeon has shown good suitability for aquaculture due to its metabolic regulatory ability to respond to acute temperature changes.
分类号:
- 相关文献
作者其他论文 更多>>
-
Examination of the relationship of carbonate alkalinity stress and ammonia metabolism disorder-mediated apoptosis in the Chinese mitten crab, Eriocheir sinensis: Potential involvement of the ROS/MAPK signaling pathway
作者:Tao, Shengqiang;Zhao, Zhigang;Tao, Shengqiang;Li, Xiaojing;Wang, Jingyao;Bai, Yichen;Wang, Jiahao;Yang, Yuhong;Zhao, Zhigang
关键词:Alkalinity stress; Eriocheir sinensis; Oxidative stress; ROS/MAPK pathway; Apoptosis
-
GhTPPA_2 enhancement of tobacco sugar accumulation and drought tolerance
作者:Wei, Yunxiao;Khan, Muhammad Aamir;Liang, Chengzhen;Meng, Zhigang;Wang, Yuan;Guo, Sandui;Zhang, Rui;Song, Yuhan
关键词:Trehalose-6-phosphate phosphatase; Drought stress; Transgenic tobacco; GhTPPA_2
-
Development of a rapid and sensitive reverse transcription real-time quantitative PCR assay for detection and quantification of grass carp reovirus II
作者:Li, Jiahao;Wang, Yajun;Wang, Yingying;Li, Yingying;Shi, Cunbin;Mo, Xubing;Wang, Qing;Yin, Jiyuan;Wu, Huiliang;Xu, Wei;Wang, Hao;Bergmann, Sven M.;Wang, Qing;Yin, Jiyuan
关键词:GCRV-II; Quantitative analysis; RT-qPCR; Detection
-
Effects of solid-state fermentation with Bacillus subtilis Y4 on the quality of Yibin Yacai
作者:Zou, Yuke;Zhong, Yixin;Zhou, Qian;Chen, Qian;Wu, Yanping;Zhong, Kai;Gao, Hong;Jia, Zhenchao;Xu, Wei;Wei, Shugu
关键词:Yibin Yacai; Bacillus subtilis Y4; Solid-state fermentation; Flavor -related metabolites; Microbial communities
-
Immune and intestinal microbiota responses to heat stress in Chinese mitten crab (Eriocheir sinensis)
作者:Li, Zhiqiang;Yang, Yuhong;Zhao, Zhigang;Luo, Liang;Wang, Shihui;Zhang, Rui;Guo, Kun
关键词:Eriocheir sinensis; Heat stress; Immune function; Intestinal microbiota
-
Influences of subcellular Ca redistribution induced by ? irradiation on the fruit firmness of refrigerated blueberries
作者:Wang, Chen;Tao, Ye;Han, Yanqiu;Zhang, Rui;Li, Lifeng;Gao, Ya
关键词:? Irradiation; ?Bluecrop ? blueberry; Fruit firmness; Subcellular Ca distribution; Ca2+signal
-
Metabolomics analysis reveals the response mechanism to carbonate alkalinity toxicity in the gills of Eriocheir sinensis
作者:Zhang, Rui;Zhao, Zhigang;Li, Mingshuai;Luo, Liang;Wang, Shihui;Guo, Kun;Xu, Wei;Li, Mingshuai;Zhang, Rui;Zhao, Zhigang;Luo, Liang;Wang, Shihui;Guo, Kun;Xu, Wei;Zhao, Zhigang
关键词:Eriocheir sinensis; Gills; Carbonate alkalinity; Metabolomics