Molecular Characterization and Expression Analysis of SKIV Infection of Interferon-Induced Protein with Tetratricopeptide Repeats 1 (IFIT1) in Epinephelus lanceolatus

文献类型： 外文期刊

第一作者： Wang Lei

作者： Wang Lei;Ma Teng;Xu Wenteng;Chen Zhangfan;Zhou Qian;Zheng Guiliang;Chen Songlin;Ma Teng;Zheng Guiliang

作者机构：

关键词： interferon-induced protein with tetratricopeptide repeats 1 (IFIT1); Epinephelus lanceolatus; tetratricopeptide repeats (TPR) motif; expression pattern; antiviral function

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）

ISSN： 1672-5182

年卷期： 2021 年 20 卷 2 期

页码：

收录情况： SCI

摘要： Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), also known as interferon-induced protein 56 (IFI56) or Interferon-stimulated protein 56 (ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1 (ELIFIT1) gene was cloned for the first time. The complete cDNA of ElIFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid (AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus (SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.

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