The CBL-Interacting Protein Kinase NtCIPK23 Positively Regulates Seed Germination and Early Seedling Development in Tobacco (Nicotiana tabacum L.)
文献类型: 外文期刊
第一作者: Shi, Sujuan
作者: Shi, Sujuan;An, Lulu;Mao, Jingjing;Aluko, Oluwaseun Olayemi;Ullah, Zia;Xu, Fangzheng;Liu, Guanshan;Liu, Haobao;Wang, Qian;Shi, Sujuan;An, Lulu;Mao, Jingjing;Aluko, Oluwaseun Olayemi;Ullah, Zia;Xu, Fangzheng;Shi, Sujuan
作者机构:
关键词: CIPK23; seed germination; cotyledon expansion; hypocotyl elongation; seedling growth; tobacco
期刊名称:PLANTS-BASEL ( 影响因子:3.935; )
ISSN:
年卷期: 2021 年 10 卷 2 期
页码:
收录情况: SCI
摘要: CBL-interacting protein kinase (CIPK) family is a unique group of serine/threonine protein kinase family identified in plants. Among this family, AtCIPK23 and its homologs in some plants are taken as a notable group for their importance in ions transport and stress responses. However, there are limited reports on their roles in seedling growth and development, especially in Solanaceae plants. In this study, NtCIPK23, a homolog of AtCIPK23 was cloned from Nicotiana tabacum. Expression analysis showed that NtCIPK23 is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. The transcriptional level of NtCIPK23 changes rapidly and spatiotemporally during seed germination and early seedling growth. To study the biological function of NtCIPK23 at these stages, the overexpressing and CRISPR/Cas9-mediated knock-out (ntcipk23) tobacco lines were generated. Phenotype analysis indicated that knock-out of NtCIPK23 significantly delays seed germination and the appearance of green cotyledon of young tobacco seedling. Overexpression of NtCIPK23 promotes cotyledon expansion and hypocotyl elongation of young tobacco seedlings. The expression of NtCIPK23 in hypocotyl is strongly upregulated by darkness and inhibited under light, suggesting that a regulatory mechanism of light might underlie. Consistently, a more obvious difference in hypocotyl length among different tobacco materials was observed in the dark, compared to that under the light, indicating that the upregulation of NtCIPK23 contributes greatly to the hypocotyl elongation. Taken together, NtCIPK23 not only enhances tobacco seed germination, but also accelerate early seedling growth by promoting cotyledon greening rate, cotyledon expansion and hypocotyl elongation of young tobacco seedlings.
分类号:
- 相关文献
作者其他论文 更多>>
-
QTL mapping and candidate gene analysis of microspore embryogenesis in Capsicum
作者:Bai, Jingyi;Chen, Bin;Qi, Meixia;Du, Heshan;Geng, Sansheng;Zhang, Xiaofen;Qi, Meixia;Wang, Qian
关键词:Pepper; Microspore embryogenesis; QTL mapping; SLAF-seq
-
SmCSP4 from aphid saliva stimulates salicylic acid-mediated defence responses in wheat by interacting with transcription factor TaWKRY76
作者:Zhang, Yong;Liu, Xiaobei;Fan, Jia;Liu, Huan;Wang, Qian;Sun, Yu;Zhang, Yumeng;Chen, Julian;Fu, Yu;Francis, Frederic;Wang, Qian;Zhang, Yumeng
关键词:grain aphid; chemosensory protein; salivary elicitor; salicylic acid; plant immunity; molecular basis
-
Lignocellulose Degradation Efficiency of Agaricus bisporus Strains Grown on Wheat Straw-Based Compost
作者:Wang, Qian;Xiao, Tingting;Juan, Jiaxiang;Zhang, Jinjing;Chen, Hui;Huang, Jianchun;Qian, Wubing;Shen, Xinfen
关键词:Agaricus bisporus; A15 and W192; substrate utilization; enzyme activity
-
Responses of differentially expressed proteins and endogenous hormones in winter rapeseed (Brassica rapa L.) roots under water deficit stress
作者:Mi, Chao;Wang, Qian;Sun, Chao;Zhang, Yusong;Zhou, Chao;Liu, Youmin;Lin, Liangbin;Zhao, Yanning;Wang, Qian;Liu, Zigang
关键词:differentially expressed protein; root system architecture; signal transduction; water deficit; winter rapeseed (Brassica rapa L; )
-
Detection distribution of CNVs of SNX29 in three goat breeds and their associations with growth traits
作者:Wang, Qian;Bi, Yi;Pan, Chuanying;Song, Xiaoyue;Zhu, Haijing;Song, Xiaoyue;Zhu, Haijing;Wu, Xianfeng;Guo, Zhengang;Liu, Mei
关键词:sorting nexin 29 (SNX29) gene; copy number variation (CNV); growth traits; goats; marker-assisted selection (MAS)
-
Whole-Genome Methylation Sequencing of Large Yellow Croaker (Larimichthys crocea) Liver Under Hypoxia and Acidification Stress
作者:Yue, Yanfeng;Wang, Yabing;Zhang, Bianbian;Zeng, Jiao;Wang, Qian;Wang, Cuihua;Peng, Shiming
关键词:Large yellow croaker; Larimichthys crocea; Hypoxia; Acidification; Liver; Methylation
-
Toxicity, baseline of susceptibility, detoxifying mechanism and sublethal effects of chlorogenic acid, a potential botanical insecticide, on Bemisia tabaci
作者:Wang, Ran;Zhang, Qinghe;Qu, Cheng;Wang, Qian;Luo, Chen;Wang, Jinda
关键词:Bemisia tabaci; chlorogenic acid; botanical insecticide; metabolic enzymes; cytochrome P450 monooxygenases; sublethal effects