Expression and functionality of allergenic genes regulated by simulated gastric juice in Anisakis pegreffii
文献类型: 外文期刊
第一作者: Wang, Xu
作者: Wang, Xu;Jia, Haiyan;Gong, Haiyan;Zhang, Yan;Mi, Rongsheng;Zhang, Yehua;Han, Xiangan;Chen, Zhaoguo
作者机构:
关键词: Anisakis pegreffii; Allergenic genes; Transcriptomic analysis; Gastric juice
期刊名称:PARASITOLOGY INTERNATIONAL ( 影响因子:2.23; 五年影响因子:2.281 )
ISSN: 1383-5769
年卷期: 2021 年 80 卷
页码:
收录情况: SCI
摘要: To better understand the molecular mechanisms underlying allergens and parasite immunity and discover the stage-enriched gene expression of fish-borne zoonotic nematodes in the stomach, we used RNA-seq to study the transcriptome profiles of Anisakis pegreffii (Nematoda: Anisakidae, AP) in simulated gastric juice. Mobile L3 larvae were incubated in simulated medium at 37 degrees C in 5% CO2 (AP-GJ) and the control group larvae were collected in PBS under the same conditions (AP-PBS). We found that the sequences of A. pegreffii were highly similar to Toxocara canis sequences. Among the transcripts, there would be 138 up-regulated putative genes and 251 down-regulated putative genes in AP-GJ group. Several lipid binging-related genes were more highly expressed in AP-GJ larvae. Moreover, 17 allergen genes were up-regulated and 29 were down-regulated in AP-GJ larvae. Eleven allergen genes belonged to one or more of the following three categories: biological process, cellular component, and molecular function. According to KEGG analysis, the main pathways that were represented included protein processing in transcription, immune system, cancer, and infectious disease. In particular, the most significant changes in the expression of parasite-derived allergen products occurred in AP-GJ larvae. This study helps us to extend our understanding of the biology of the fish-borne zoonotic parasite A. pegreffii and could be helpful for more precise risk assessment and providing guidelines for allergic consumers.
分类号:
- 相关文献
作者其他论文 更多>>
-
Fibrous whey protein mediated homogeneous and soft-textured emulsion gels for elderly: Enhancement of bioaccessibility for curcumin
作者:Wang, Zhiming;Deng, Yuanyuan;Zhang, Yan;Tang, Xiaojun;Zhou, Pengfei;Li, Ping;Zhao, Zhihao;Liu, Guang;Zhang, Mingwei;Wang, Zhiming;Zhang, Mingwei;Zhang, Mingwei;Wang, Zhangying
关键词:Protein self-assembly aggregates; Oil-water state; Texture properties; Gelation dynamic; Macrophase separation
-
Quantifying key vegetation parameters from Sentinel-3 and MODIS over the eastern Eurasian steppe with a Bayesian geostatistical model
作者:Li, Zhenwang;Liu, Tao;Sun, Chengming;Li, Zhenwang;Liu, Tao;Sun, Chengming;Ding, Lei;Shen, Beibei;Xu, Dawei;Wang, Xu;Xin, Xiaoping;Chen, Jiquan;Fang, Wei;Pulatov, Alim;Kussainova, Maira;Amarjargal, Amartuvshin;Isaev, Erkin
关键词:Biophysical parameter; Remote sensing; Bayesian modeling; Sentinel-3; MODIS; Grassland
-
TMT-based proteomic and transcriptomic analysis reveal new insights into heat stress responsive mechanism in edible mushroom Grifola frondosa
作者:Xie, Hongyan;Wan, Luzhang;Han, Jiandong;Huang, Chunyan;Li, Jin;Yao, Qiang;Yang, Peng;Gong, Zhiyuan;Zhang, Yan;Yu, Hao
关键词:Grifola frondosa; Proteomics; Heat stress response; Transcriptomics; Heat shock protein
-
Semi-closed synthesis of mesoporous carbon supported metal oxide nanoparticles
作者:Xing, Chen;Zhang, Shiguo;Zhang, Yan;Xing, Chen;Tan, Zhijian
关键词:Semi-closed synthesis; Mesoporous carbon; Metal oxide nanoparticle; Selective aqueous oxidation
-
Application of Chlorogenic acid as a substitute for antibiotics in Multidrug-resistant Escherichia coli -induced mastitis
作者:Feng, Shiyuan;Zhang, Yihao;Fu, Shaodong;Li, Zhi;Zhang, Jinqiu;Xu, Yuanyuan;Miao, Jinfeng;Feng, Shiyuan;Han, Xiangan
关键词:Chlorogenic acid; Escherichia coli; Mastitis; Antimicrobial; Antioxidant; Anti-inflammatory
-
Natural variation in SlSOS2 promoter hinders salt resistance during tomato domestication
作者:Wang, Xu;Yu, Shuojun;Wang, Zhiqiang;Wang, Zhen;Hong, Yechun;Kong, Dali;Yu, Yongdong;Chao, Zhen-Fei;Liu, Xue;Huang, Sanwen;Wang, Zhen;Hong, Yechun;Kong, Dali;Yu, Yongdong;Chao, Zhen-Fei;Liu, Xue;Zhu, Jian-Kang;Wang, Zhen;Guan, Xijin;Zhu, Guangtao;Huang, Sanwen;Zhu, Jian-Kang;Zhu, Jian-Kang
关键词:
-
Insight into muscle quality of white shrimp (Litopenaeus vannamei) frozen with static magnetic-assisted freezing at different intensities
作者:Sun, Qinxiu;Zhang, Honghong;Hou, Qian;Zhang, Yan;Su, Jiangpeng;Liu, Xianhua;Wei, Qihang;Ji, Hongwu;Liu, Shucheng;Yang, Xianqing;Sun, Qinxiu;Liu, Shucheng;Dong, Xiuping
关键词:White shrimp; Magnetic field-assisted immersion freezing; Muscle quality; Ice crystal