Mapping of the Waxy Gene in Brassica napus L. via Bulked Segregant Analysis (BSA) and Whole-Genome Resequencing
文献类型: 外文期刊
第一作者: Zhang, Junying
作者: Zhang, Junying;Zhu, Jifeng;Yang, Liyong;Li, Yanli;Wang, Weirong;Zhou, Xirong;Jiang, Jianxia
作者机构:
关键词: Brassica napus L; bulked segregant analysis; whole-genome resequencing; waxy; waxless
期刊名称:AGRONOMY-BASEL ( 影响因子:3.7; 五年影响因子:4.0 )
ISSN:
年卷期: 2023 年 13 卷 10 期
页码:
收录情况: SCI
摘要: Plant cuticular wax is the covering of the outer layer of the plant. It forms a protective barrier on the epidermis of plants and plays a vital role like a safeguard from abiotic and biotic stresses. In the present study, Brassica napus L. materials with and without wax powder were observed. Genetic analysis showed that the separation ratio of waxy plants to waxless plants was 15:1 in the F2 population, which indicated that the wax powder formation was controlled by two pairs of genes. In order to identify the candidate genes associated with the wax powder trait of B. napus L., bulked segregant analysis (BSA) was performed. The homozygous waxy plants, the homozygous waxless plants, and plants from three parents were selected for establishing five DNA pools for genome-wide resequencing. The results of the resequencing showed that the site associated with wax powder trait was located in the region of 590,663-1,657,546 bp on chromosome A08. And 48 single nucleotide polymorphisms (SNPs) were found between the DNA sequences of waxy plants and waxless plants in this region. These SNPs were distributed across 16 gene loci. qRT-PCR analysis was conducted for the 16 candidate genes and three genes (BnaA08g01070D, BnaA08g02130D, and BnaA08g00890D) showed significantly differential expression between waxy and waxless parents. BnaA08g01070D and BnaA08g02130D were significantly down-regulated in the waxless parent, while BnaA08g00890D was significantly up-regulated in the waxless parent. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that the BnaA08g02130D gene was enriched in lipid biosynthetic or metabolic processes. All the results in our study would provide valuable clues for exploring the genes involved in wax powder development.
分类号:
- 相关文献
作者其他论文 更多>>
-
Effects of Different Tillage Practices on Soil Stability and Erodibility for Red Soil Sloping Farmland in Southern China
作者:Jin, Huifang;Li, Junkai;Li, Jifu;Li, Yanli;Zhu, Hai;Jin, Huifang;Li, Junkai;Li, Jifu;Li, Yanli;Zhu, Hai;Huang, Shangshu;Shi, Dongmei
关键词:tillage method; soil aggregate stability; wet aggregate stability; mechanical soil stability; soil erodibility; red soil hilly region
-
QTL mapping for seed density per silique in Brassica napus
作者:Zhu, Jifeng;Lei, Lei;Wang, Weirong;Jiang, Jianxia;Zhou, Xirong
关键词:
-
Soil nitrogen dynamics in natural forest ecosystem: a review
作者:Sardar, Muhammad Fahad;Li, Yanli;Sardar, Muhammad Fahad;Younas, Fazila;Farooqi, Zia Ur Rahman
关键词:forest ecosystem; bamboo invasion; nitrogen cycling; broadleaf forests; soil properties
-
Protection against Metabolic Associated Fatty Liver Disease by Protocatechuic Acid
作者:Tan, Jijun;Hu, Ruizhi;Gong, Jiatai;Fang, Chengkun;Li, Yanli;He, Jianhua;Wu, Shusong;Liu, Ming;He, Ziyu;Hou, De-Xing;Zhang, Hongfu;Wu, Shusong
关键词:Enterococcus faecalis; nonalcoholic fatty liver disease; insulin; protocatechuic acid; gut microbiota
-
The ZmbHLH32-ZmIAA9-ZmARF1 module regulates salt tolerance in maize
作者:Yan, Zhenwei;Wang, Wenli;Leng, Bingying;Yao, Guoqi;Zhang, Fajun;Mu, Chunhua;Liu, Xia;Li, Ke;Li, Yanli
关键词:Signal transduction; Maize; Salt stress
-
Current Status and Spatiotemporal Evolution of Antibiotic Residues in Livestock and Poultry Manure in China
作者:Li, Yanli;Li, Qingjie;Ji, Zhengyu;Andom, Okbagaber;Wang, Xiaoxing;Guo, Xueqi;Li, Zhaojun
关键词:antibiotic; manure of livestock and poultry; spatial distribution; livestock and poultry species
-
CRISPR/Cas9-mediated seamless gene replacement in protoplasts expands the resistance spectrum to TMV-U1 strain in regenerated Nicotiana tabacum
作者:Li, Yanli;Zhao, Shancen;Huang, Changjun;Liu, Yong;Zeng, Jianmin;Yu, Haiqin;Tong, Zhijun;Sui, Xueyi;Fang, Dunhuang;Xiao, Bingguang;Yuan, Cheng;Yuan, Xinjie
关键词:protoplast regeneration; gene knock-in; gene replacement; CRISPR/Cas9; long fragment insertion; TMV resistance